我国肝病病人和健康人群中TTV基因型分析

Analysis of TTV genotype in hepatitis patients and healthy volunteers in China

目的 分析我国肝病患者和健康人群TTV基因型。方法 用巢式PCR扩增我国不同地区不明病因的急性肝炎、慢性肝炎、肝硬化、肝癌和自然健康人群TTV DNA片段，并对PCR产物进行克隆，测序分析和同源性比较。结果 武汉WH1、WH2、WH3 3个病人血清中TTV DNA序列与N22同源性分别为97%、97%和98%；广州GZ 1、GZ2、GZ3 3例病人血清中TTV DNA 序列与N22同源性分别为98%、95%和95%；山东SD2、SD3 TTV DNA序列与N22同源性分别为94.6%和95.5%；广州GZ4、山东SD1、新疆XJ1病人TTV DNA序列与TXO11同源性分别为98%、98%和95%。结论 根据 Okamoto分类方法，我国TTV属于两个亚型，即基因1型中的a和b亚型。

Objective To analysis of TTV genotype/subtype in hepatitis patients and healthy volunteers in China. Methods DNA fragment of TTV was amplified by polymerase chain reaction with nested primers in 8 patients with liver disease and 3 healthy persons in China. The nested PCR products was cloned and sequenced. Results A TTV DNA sequence of 222 bp (primer sequence excluded) was prepared from 11 persons. The homology between N22 and WH1,WH2,WH3,GZ1,GZ2,GZ3,SD2,SD3 respectively was 97%, 97%, 98%, 98%, 95%, 95%,.94.6% and 95.5%.The homology between TXO11 and GZ4,SD1,XJ1 respectively was 98%，98% and 95%. Conclusion According to Okamoto's methods, the 11 TTV clones were classified into genotype/subtype 1a and 1b.