摘要
目的：探讨p21(WAF1/CIP1)基因mRNA及p21WAF1蛋白、PCNA（增殖细胞核抗原）与骨肉瘤的发生、发展之间的关系及其对预后的评价。方法：采用原位杂交及免疫组化法（LSAB法）检测p21(WAF1/CIP1)基因mRNA及p21WAF1蛋白、PCNA在骨肉瘤中的表达。结果：原位杂交结果显示，p21(WAF1/CIP1)mRNA在45例骨肉瘤中有19例阳性表达，阳性率为42％；在10例骨纤维结构不良中有8例阳性表达，阳性率为80％。免疫组化结果显示，p21(WAF1)蛋白在45例骨肉瘤中有8例阳性表达，阳性率为17.7％；在10例骨纤维结构不良有5例阳性表达，阳性率为50％。PCNA在45例骨肉瘤中均有表达，阳性率为100％。p21(WAF1/CIP1)mRNA表达阳性者术后生存时间与p21(WAF1/CIP1)mRNA表达阴性者术后生存时间之间的差异有显著性（P<0.05）。结论：①随着骨肿瘤恶性度的升高，p21(WAF1/CIP1)mRNA及p21WAF1蛋白的表达下降；②p21(WAF1/CIP1)mRNA及p21WAF1蛋白表达的数量和水平的下降是导致人类骨组织发生恶性骨肿瘤的可能原因之一；③随访结果显示，p21(WAF1/CIP1)mRNA在骨肉瘤中的表达对预后有影响。
Objective： To investigate the expressions of protein p21WAF1/CIP1 and PCNA in human osteosarcoma, and their relationships to the development and progress of osteosarcoma the assess of their prognosis of the patient. Methods： The expressions at mRNA level and protein level for p21WAF1/CIP1 and PCNA were determined in 45 cases of osteosarcoma by using in stita hybridization and immunohistochemical techniques respectively. Results： The positive rate of p21WAF1/CIP1 at mRNA level in osteosarcoma and fibro osseous dysplasia was 42％ and 80％ respectively (P< 0.05). The positive rate of p21WAF1 protein between osteosarcoma and fibro osseous dysplasia ( 17.7％ and 50％ respectively) was also significantly different(P< 0.05) . while positive rate of PCNA was 100％ . There was significant difference of survival time after operation between patient with p21WAF1/CIP1 mRNA positive expression and that with p21WAF1/CIP1 mRNA negative expression(P< 0.05). Conclusions： ① Along with increase of malignancy the expressions of p21WAF1/CIP1 mRNA and p21WAF1 protein in bone tumor tend to decrease; ② The decrease of p21WAF1/CIP1 mRNA and p21WAF1 protein expression may be one of the causes induceing maligant tumor in bone;③ The follow up resuts indicate that the exression of p21WAF1/CIP1 mRNA in osteosarcoma can influence the prognosis of osteosarcoma patients.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第9期850-855,共6页
Chinese Journal of Cancer
基金
本课题受卫生部科学研究基金项目!(96-1-121)
