一种新的乳腺癌相关抗原的初步分离纯化与鉴定

Separation and characterization of a new breast tumor associated antigen

目的：分离纯化及鉴定一种新的乳腺癌相关抗原。方法：将乳腺癌组织粗提取物进行DEAE－纤维素阶段洗脱，经ELISA法检测其特异性蛋白峰，通过SDS－PAGE电泳和ELISA方法进一步纯化及鉴定该抗原，并用酶法、热稳定性检测其理化特性。结果：洗脱的3个蛋白峰中，峰1与乳腺癌患者血清中抗体反应，不与健康妇女、良性乳腺疾病和其它肿瘤患者的血清反应。乳腺癌相关抗原存在于峰1中，分子量为85kDa，为糖蛋白。结论：该抗原在乳腺癌的诊断及判断预后方面具有一定的价值。

Objective： To separate and characterize a new breast tumor associated antigen. Methods： The specific protein peak was obtained by DEAE cellulose discontinuous chromatography of a crude extract of breast tumor and tested by ELISA.The breast tumor associated antigen was purified further by SDS PAGE and tested by ELISA.And the physico chemical characterization was determined by testing it s enzyme susceptibility and thermostability.Results： Circulating antibodies against first protein peak were observed in breast cancer patients and absent in patients with carcinoma of other organs or with benign breast disease and in healthy women. The breast tumor associated antigen was found in the first protein peak with molecular weight of approximately 85 kDa and characterized as a glycoprotein. Conclusion： The antigen appears to be a potential diagnostic and prognostic marker for breast cancer.