利用EROD和GST酶评估竹子制浆造纸废水的生物毒性

Assessing Biological Toxicity of Bamboo Pulp and Paper Effluent by Determining EROD and GST Activity in Mosquitofish

测定了生活于受竹子制浆造纸废水(采用"小苏打浸泡法"制浆)污染的河道中食蚊鱼活体肝脏7-乙氧基-3-异吩呛哇酮-脱乙基酶(7-ethoxyresorufino-deethylase,EROD)和谷胱甘肽硫转移酶(Glutathione-S-transferase,GST)的活性,并论证了食蚊鱼作为评价竹子制浆造纸废水生物毒性的指示生物的可行性。结果表明,食蚊鱼是比较理想的评价竹子制浆造纸废水生物毒性的指示生物,其活体EROD能有效、真实地评价竹子制浆造纸废水的安全性,GST在指示竹子制浆造纸废水生物毒性时的效果不如EROD。

The biological toxicity of bamboo pulp and paper effluent was assessed by determining EROD and GST activity in mosquitofish（Gambusia affinis） exposed to the effluent.The result showed that both female and male mosquitofish exposed to the effluent had significantly higher levels of EROD activity than that of mosquitofish living in the site which had no known point sources of pollution.Mosquitofish was a suitable aquatic animal to evaluate bamboo pulp and paper effluent pollution effects.EROD activity was a more sensitive indicator than the GST activity to monitoring of bamboo pulp and paper effluent contamination.