四倍体沙01梨F_1代染色体数目鉴定

Observation on Chromosome Number of F_1 Hybrid Progeny of Tetraploid Pear Cultivar ‘Sha 01’

本文对四倍体沙 0 1梨 F1 代单株进行了染色体数目鉴定 ,共 8个组合 1 0 7株 ,发现4 9株三倍体 2 n=3 x=51 ,其中 1株为大水核×沙 0 1 F1 代 ,2 6株为沙 0 1×大南果梨 F1 代 ,3株为大南果梨×沙 0 1 F1 代 ,1 1株为沙 0 1×秦酥 F1 代 ,2株为安梨×沙 0 1 F1 代 ,6株为沙 0 1×金花 4号 F1 代 ,三倍体出现率为 4 5.8 ;1株为四倍体 2 n=4 x=68 ,系安梨×沙 0 1 F1 代 ,占观察总数的 0 .9 ;1 0株为非整倍体 ,均为三倍体作母本的 F1 代 ,占观察总数的 9.3 .结果表明 ,沙 0 1梨作母本 ,二倍体大南果梨作父本 ,是获得三倍体单株的理想组合 ,沙 0

Chromosome numbers of 107 F 1 hybrid seedlings derived from 8 hybridized combinations of tetraploid pear cultivar ‘Sha 01’ as seed or pollen parent were observed and it was found that 49 seedlings,including 1 seedling from Dashuihe×Sha 01,26 from Sha 01×Dananguo ,3 from Dananguo×Sha 01,11 from Sha 01×Qinsu ,2 from Anli ×Sha 01,6 from Sha 01×Jinhua 4,were triploid (2n=3 x =51) and the ratio of triploid was 45.8%; 1 seedling from Anli× Sha 01 was tetraploid (2 n =4 x =68) and the ratio of tetraploid was 0.9%;10 seedlings,all from triploid pear as seed parent ,were aneuploid and the ratio of aneuploid was 9.3%.The results show that hybridized combination of Sha 01 pear as seed parent and Dananguo pear as pollen parent is the ideal combination for obtaining triploid hybrid seedlings and Sha 01 pear is the excellent germplasm resource for polyploid pear breeding.