草鱼出血病病毒854株基因组SDS—PAGE分析及其核酸类型鉴定

Genome SDS—PAGE analysis and nucleic acid identification of grass carp hemorrhage virus strain 854

十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)结果表明:草鱼出血病病毒854株基因组由11个分段的核酸片段组成,总分子量约为14.46×10~6道尔顿。其中最大片段的分子量约为2.45×10~6道尔顿;最小片段的分子量约为0.38×10~6道尔顿。其基因组核酸片段电泳图谱具有病毒株的特异性.核酸酶敏感性试验表明该病毒的核酸为RNA。

SDS-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the genome of grass carp hemorrhage virus strain 854 was composed of 11 segments of dsRNA The molecular weight of the segments ranged from 2. 45×10~6 to 0. 38×10~6 daltons with a total genome of approximately 14. 46×10~6 daltons The electrophoresis pattern of the viral genome has strain-specific characteristics The sensitivity test to nuclaase revealed that the viral nucleic acid is RNA.