胆脂瘤型中耳炎细菌检测及耐药性分析

Type Cholesteatoma Otitis Media BacteriaDetection and Drug Resistance Analysis

目的探讨胆脂瘤型中耳炎患者细菌感染的菌种分布及耐药性发生情况,为临床合理使用抗生素对抗胆脂瘤型中耳炎感染提供参考。方法选择74例到该院就诊的胆脂瘤型中耳炎患者耳部流脓标本进行培养,获得患者致病菌组成分布。对获得的菌株进一步进行生物膜半定量检测和药敏试验检测,分析其耐药性。结果所有观察对象中所获得的胆脂瘤型中耳炎致病菌从高到低分布依次为金黄色葡萄球菌、铜绿假单胞菌、表皮葡萄球菌,有生物膜的细菌普遍比无生物膜的细菌耐药率高。结论胆脂瘤型中耳炎致病菌发生率高低可因"三间"不同而有差异,并且有生物膜的细菌比无生物膜的细菌耐药率高,值得临床注意。

Objective To detect the bacterial species distribution and drug resistance of occurrence of chronic otitis media with cholesteatoma, and to provide reference for clinical medication of chronic otitis media with cholesteatoma. Methods Media pus from 74 cases with chronic otitis media with cholesteatoma from 2010 June to 2012 May were collected and cultrured to acquire pathogen composition distribution. The obtained strains were further biofilm semi quantitative detection and drug sensitivity test, to analys their drug resistance. Results Staphylococcus, Pseudomonas aeruginosa, Staphylococcus aureus were the most frequently insolated pathogens in the observed objects in acquired cholesteatoma otitis media pathogens. Resistance rate with biofilm bacteria was higher than that without biofilm bacterial. Conclusion Cholesteatoma otitis media pathogens is different due to ＂ three ＂, and bacterial resistance rate is higher with biofilm than without biofilm. which are worthy of attention and in-depth analysis in clinical practice.