浊点萃取、荧光淬灭法测定痕量维生素B2

Determination of trace vitamin B2 by fluorescence quenching after cloud point extraction

根据高聚物的强富集作用及荧光淬灭法灵敏度高等特点,提出了浊点萃取、荧光淬灭法测定痕量维生素B2的新方法。主要研究了萃取剂、萃取时间、酸度、铬蓝黑的浓度、淬灭时间等因素的影响,并探讨了利用维生素B2淬灭铬蓝黑R的机理。实验表明,在最佳条件下,聚乙二醇辛基苯基醚～硫酸铵体系对维生素B2的一次萃取率可达到98%,利用该方法测定维生素B2的线性范围为:1.2×10-7～1.44×10-5g/L;回归方程:ΔF=0.13+3.0C;相关系数为0.9974;检出限:8.4×10-8g/L。该方法用于样品测定,相对标准偏差在2.9%～5.8%之间。

Based on the strong accumulation of polymer synthesis and the high sensitivity of fluorescence quenching etc, a new method for the determination of trace vitamin B2by fluorescence quenching after aqueous two-phase extraction was proposed. The paper discussed various factors such as the extractant, extraction time, acidity, the concentration of chromium nerazzurri R, quenching time etc, and studied the mechanism of fluorescence quenching of chromium nerazzurri R by vitamin B2. The results indicated that the extraction efficiency of vitamin B2 in the Polyethylene glycol octyl phenyl ether - （NH4）ESO4system was 98% in one-step extraction under the optimal conditions. The linear range of vitamin B2 was 1.2× 10-7-1.44- 10-Sg/L, with regression equation： AF = 0. 13 ＋ 3.0 C; and the correlation coefficient was 0. 9974; the detection limit was 8.4-10- s g/L; and the relative standard deviations were 2.9% -5.8 %. Compared with other analysis for vitamin BE, this method has characteristics such as low detection limit, wide linear range, low-toxic, pro- environment etc.