HPLC法测定跌打巴布剂中三七皂苷R1和人参皂苷Rg1含量

HPLC Determination of Notoginsenoside R1 and Ginsenoside Rg1 in Dieda Cataplasm

目的建立跌打巴布剂中三七皂苷R1和人参皂苷Rg1的含量测定方法。方法采用HPLC法,以Agilent TC-C18ODS柱为色谱柱,以乙腈-水（24：76）为流动相,检测波长203nm,流速1mL/min。结果三七皂苷R1线性范围为0.583~29.15μg（r=0.9999,n=5）,回收率为99.9%~100.3%。人参皂苷Rg1线性范围为0.336~16.8μg（r=0.9999,n=5）,回收率为99.8%~100.2%。结论本方法准确、可靠、灵敏度高,可用于跌打巴布剂的定量检测。

Objective Determine notoginsenoside R1 and ginsenoside Rg1 in Dieda cataplasm by HPLC.Methods Agilent TC-C18 ODS column was used with the mobile phase containing acetonitrile-water（24：76）.The wavelength of detector was set at 203nm.The flow rate was 1mL/min.Results The calibration curves were linear in the range of 0.583~29.15μg for notoginsenoside R1（r=0.9999,n=5） and 0.336~16.8μg for ginsenoside Rg1（r=0.9999,n=5）.The recovery of notoginsenoside R1 was 99.9%~100.3%,and that of ginsenoside Rg1 was 99.8%~100.2%.Conclusion The method is accurate,reliable and simple,and is suitable for the determination of Dieda cataplasm.