HPLC法再研究水煎煮时间对柴胡皂苷成分溶出量的影响

HPLC method of water decocting time affect the amount of saikosaponin ingredients

目的:HPLC法再研究水煎煮时间对柴胡皂苷成分溶出量的影响。方法:采用Purospher STAR C18色谱柱(4.6mm×250mm,5μm);流动相:甲醇-0.05%磷酸(78∶22);流速:1.0ml.min-1;柱温:35℃;检测波长为210nm。结果:柴胡皂苷a、柴胡皂苷d线性范围分别为0.04192～20.96μg,0.03218～16.09μg;相关系数:r≥0.9998;平均回收率分别99.08%、98.27%,RSD分别为0.40%、0.55%。结论:所建立的方法简便、稳定,很好地测定了水煎煮柴胡0.5、1、2、4、6、8、10、12小时后柴胡皂苷成分溶出量,并表明水煎煮8小时柴胡制剂中柴胡皂苷依然有比较多的剩余量,在临床应用中依然应该关注其使用的安全性。本试验结果有助于制定柴胡毒性物质基础的安全范围及功效物质基础发挥药效作用的最低限度,最大限度保证柴胡临床用药的安全、有效。

Objective： Tto study HPLC method and decoction time affect the amount of saikosaponin ingredients.Methods： the Purospher STAR C 18 column（4.6mm ×250mm,5μm）;mobile phase： methanol-0.05% phosphate（78 ∶22）;flow rate： 1.0ml.min-1;column temperature： 35℃;the detection wavelength was 210nm.Results： the saikosaponin a,saikosaponin d linear range was 0.04192 ～ 20.96μg,0.03218～16.09μg;Correlation coefficient：r≥0.9998;the average recoveries were 99.08%,98.27%,RSD were 0.40%,0.55%.Conclusion： the established method is simple,stable,good determination of water decocting Bupleurum 0.5,1,2,4,6,8,10,12 hours after the release of saikosaponin components,and show that the water is boiling 8 hours of Bupleurum Bupleurum preparation of saponins are still remaining amount more,in clinical application still should pay attention to the safety of their use.The results of this study have minimal help basic safety scope of Bupleurum toxic substances based and efficacy substances play a pharmacodynamics,maximum safety,effective clinical use of Radix bupleuri.