HPLC法测定长春西汀脂质体的药物含量及包封率

HPLC determination of Vinpocetine and its entrapment efficiency in nanoliposome

目的:建立长春西汀脂质体的药物含量方法。方法:采用Kromasil C18柱(4.6mm×200mm,5μm),流动相为甲醇-0.1mol.L-1碳酸铵水溶液(90∶10,v/v),检测波长314nm,柱温为室温,流速:1.0mL.min-1。结果:长春西汀在4.00～60.00μg.ml-1浓度范围内线性关系良好,峰面积对浓度有良好的线性关系,回归方程为:A=9437.5C+1534.3(r=0.9999,n=7),方法的日内与日间精密度RSD均<2%,回收率分别为101.4%、100.3%、100.5%。结论:该方法具有简便、快速、准确的特点,可用于长春西汀脂质体的药物含量及包封率测定。

Objective：To establish an RP-HPLC method for content and entrapment efficiency determination of Vinpocetine in nanoliposome.Methods：The separation was performed on a Kromasil C18column（4.6mm×200mm,5μm）at room column temperature.The mobile phase consisted of methanol-0.1mol.L-1water（90∶10）at the flow rate of 1ml.min-1.The detection wavelength was at 314 nm.Results：The linear range for Vinpocetine was in the range of 4.00～60.00μg.ml-1（r=0.9999,n=7）.The within-day and between-days variation coefficients were less than 2%.The average recoveries were 101.4%,100.3% and 100.5%,respectively.Conclusion：This method is simple,accurate,sensitive and applicable for determination of content and entrapment efficiency of Vinpocetine in nanoliposome.