珍珠菜总黄酮对白血病HL-60细胞裸鼠移植瘤的抑制作用

Effect of total flavonoids from Lysimachia clethroides Duby on human leukemia HL-60 cell xenograft in nude mice

目的研究珍珠菜总黄酮(ZE4)对白血病HL-60细胞裸鼠皮下移植瘤的抑制作用。方法成功建立人白血病HL-60细胞皮下移植瘤模型(移植瘤体积约100mm3)后的30只裸鼠均分为五组:A、B、C组分别腹腔注射ZE4 100、200、400mg/kg;D组注射环磷酰胺(CTX)50mg/kg;E组以生理盐水对照;每2天注射一次。21d后测量移植瘤大小,采用免疫组化法检测移植瘤组织中细胞增殖抗原Ki-67和血管内皮细胞膜抗原CD34的表达水平。结果与E组比较,B和C组移植瘤生长明显抑制,抑瘤率分别可达51.32%和46.06%;免疫组化结果显示,B、C组移植瘤中Ki-67和CD34的表达明显少于E组。结论 ZE4对白血病HL-60细胞裸鼠移植瘤具有较明显的抑制作用,可能与其抑制肿瘤细胞增殖和肿瘤新生血管形成相关。

Objective To investigate the effect of total flavonoids from Lysimachia clethroides Duby（ZE4） on human leukemia HL-60 cell xenograft in nude mice. Methods After HL-60 cells were inoculated subcutaneously in nude mice to establish xenograft models of leukemia in 30 mice, the mice were equally randomized into 5 groups. The mice in groups of A, B and B were intraperitoneally injected with ZE4 100,200 and 400 mg/kg, respectively, those in group D with CTX 50 mg/kg, and those in group E with normal saline as hlank controls. The injection was performed every other day for 21 days. The inhibitory effect of ZE4 was observed and immunohistochemistry was used to detect the positive expressions of Ki-67 and CD34 in the xenografts. Results Compared with group E, the growth of xenografts was significantly inhibited in groups of B and C with the inhibition rates of 51.32 % and 46.06%, respectively（P〈0. 05）. Immunohistoehemistry showed that the expressions of Ki-67 and CD34 in the xenografts in groups of B and C were obviously less than those in group E. Conclusion ZE4 has a significant inhibitory effect on leukemia, which might be related to the suppression of angiogenesis and proliferation of tumor cells.