卵巢移植对去势大鼠海马神经元数量和ER-β、NG表达的影响

The effect of ovarian transplantation on the number of hippocampal neurons and ER-β and NG expression in hippocampal neurons of ovariectomized rat

目的:观察去势大鼠卵巢移植后海马神经元数量变化及雌激素受体β(ER-β)和神经颗粒素(NG)表达的变化,评价卵巢移植对中枢神经的保护作用。方法:成年雌性SD大鼠行双侧卵巢切除术(OVX)后,在肾被膜下移植出生3日内乳鼠的卵巢,于干预后的4、7、14、21 d和28 d,通过Nissl染色计数海马神经元的数量,采用免疫组化法观察海马神经元ER-β和NG蛋白的表达情况,并与OVX组和正常对照组比较。结果:与OVX组相比,卵巢移植组随移植时间的延长,海马神经元的数量逐步恢复;ER-β和NG蛋白的表达在海马各区均有所升高(P<0.05)。结论:卵巢移植可以防止海马神经元数量的减少,并促进ER-β和NG表达,发挥雌激素对中枢神经系统的保护作用。

Objective： To observe the impact of ovarian transplantation of castrated rats on the number of hippocampal neurons and the expression of estrogen receptor （ER-β） and neurogranin （NG） in hippocampal neurons, and evaluate the protective effect of ovarian transplantation on central nervous system. Methods：Aduh female SD rats were performed ovariectomy （OVX） and then transplanted the ovaries of SD rats within 3 days under the renal capsule. 4,7,14,2l days and 28 days after ovarian transplantation, the number of hippocampal neurons and the expression of ER-β and NG were checked by Nissl staining and immunohistochemical method respectively, and compared the results with the OVX group and normal control group. Results： Compared with the OVX group, the number of hippocampal neurons gradually increased with the migration time extending, and the expression of ER-β and NG increased at every regions in the hippocampus in the ovarian transplantation group （P 〈0.05 ）. Conclusion： Ovarian transplantation can restrain the number of hippocampal neurons in the ovariectomy rats, promote the expression of ER-β and NG, and plays a protective effect of es- trogen on the central nervous system.