胚鼠真皮细胞辅助毛囊重建的实验研究

Experimental research of hair follicle reconstruction with the aid of embryonic mice dermal cells

目的探讨胚胎期真皮信号对体外扩增后的新生鼠真皮细胞毛发诱导能力的影响。方法将胎龄14d的胚鼠真皮细胞，加入到由体外扩增3d的新生鼠真皮细胞与新鲜分离的新生鼠表皮细胞在裸鼠背部构建的小室中，将其毛发再生情况与未添加胚鼠真皮细胞时做比较。同时，分别以下列细胞构建小室作为对照：胚鼠真皮细胞＋新生鼠表皮细胞；新鲜分离的新生鼠真皮细胞＋新生鼠表皮细胞；扩增的新生鼠真皮细胞；胚鼠真皮细胞；新鲜分离的新生鼠真皮细胞；新生鼠表皮细胞。结果在胚鼠真皮细胞辅助下形成的毛发数量为（207±15．948）根，明显高于缺乏胚鼠真皮细胞辅助时形成的毛发数量（67±8．963）根，差异有统计学意义（n=3，t=7．653，P=0．002）。结论胚胎期真皮信号可使体外扩增后的新生鼠真皮细胞的毛发诱导能力得到更充分的发挥。

Objective To investigate the effect of embryonic dermal signal on the hair-inductive capacity of neonatal mice dermal cells which have been amplified in vitro. Methods Embryonic mice dermal cells of embryonic day 14 were added to a chamber on the back of nude mice with neonatal mice dermal cells which had been amplified in vitro for 3 days and freshly isolated neonatal mice epidermal cells. The hair regeneration was compared between the groups with or without embryonic mice dermal cells.Meanwhile, chambers with following cells respectively were constructed as controls： embryonic mice dermal cells ＋ neonatal mice epidermal cells; freshly isolated neonatal mice dermal cells ＋ neonatal mice epidermal cells; amplified neonatal mice dermal cells only; embryonic mice dermal cells only; freshly isolated neonatal mice dermal cells only; neonatal mice epidermal cells only. Results The number of regenerated hairs with the aid of embryonic mice dermal cells（207 ± 15. 948） was significantly higher than that（67 ± 8. 963 ） in the group without embryonic mice dermal cells （ n = 3, t = 7. 653, P = 0.002）. Conclusion Embryonic dermal signal can enhance the hair-inductive capacity of neonatal mice dermal cells which have been amplified in vitro.