精子-去透明带仓鼠卵穿透试验与精液分析主要参数的关系

Relationship between the main parameters of semen analysis and zona-free hamster oocyte test

目的 了解精液分析 (SA)与精子 -去透明带仓鼠卵穿透试验 (ZHOT)检测结果的关系 ,以评价此两种实验方法对评估男性生育力的临床实用价值。方法 对 2 2例男性不育症患者作了SA和ZHOT(改良的Yanagimachi方法 )的检测 ,并对SA主要参数指标的检测结果与ZHOT的检测结果 (穿卵率 )进行统计分析。结果 ZHOT之穿卵率与SA主要参数 :精子密度、正常形态精子百分率间有显著的正相关 (P <0 0 5 ) ,与a ,b级活动精子百分率间则有非常显著的正相关 (P<0 0 1)。另外 ,以ZHOT检测结果正常与否分成之两组间其SA主要参数的检测结果间差异均有显著性 (t检验 ) ,其中a ,b级活动精子百分率之差异最为显著 (P <0 0 0 1)。结论 改良的Yamagimchi精子 -去透明带仓鼠卵穿透试验确实能比较好的综合反映精液的质量 ,但因其方法较复杂 ,设备要求较高 ,精液分析仍应是一般临床单位评估男性生育力的首选实验室方法。特别是其中精子活动力的测定对男性生育力的评估更具临床实用价值。

Objective To study the relationship between Semen analysis (SA) and zona-free hamster oocyte test (ZHOT) as well as their values in assessing fertile potential of man.Methods SA and ZHOT (improved Yangagimachi method) were measured and analyzed in 22 infertile men.Results There were significant correlations between the oocyte penetrating rate and sperm concentration( P <0 05) or the percentage of normal morphological sperm ( P <0 05). There was also significant correlation between the oocyte penetrating rate and a,b grade motile sperm concentration ( P <0 01). In addition, the comparisons between two groups of semen specimens with normal or abnormal oocyte penetrating rate ( t test) showed that there were very significant difference in all main parameters of SA, of which the difference of a,b grade motile sperm percentage was the most significant ( P <0.01).Conclusion ZHOT of improved Yanagimachi method can well reflect the comprehensive semen quality. Because of its simplicity, SA should be the first and practical selection for assessing male fertility potential, most valuable in assessing sperm motility.