N-苯基-2-萘胺对青菜生长及AsA-GSH循环影响研究

Effects of N-phenyl-2-naphthylamine on the Growth and AsA-GSH Circulation Metabolism in Chinese Cabbage

以青菜为材料,研究使用不同浓度N-苯基-2-萘胺处理后对青菜生长及其体内AsA-GSH循环的影响。结果表明,使用不同浓度N-苯基-2-萘胺对青菜的株高及生物量产生不同的影响,其中以0.50 mg/L的处理效果最好,当浓度大于0.50 mg/L时,株高和生物量均随着N-苯基-2-萘胺使用浓度的增加而降低;处理后还原型抗坏血酸(AsA)、脱氢抗坏血酸(DHA)、总量Vc和AsA/DHA比值的变化趋势相似,0.50 mg/L处理下,AsA、DHA、总量Vc、AsA/DHA比值与对照相比均显著增加,当浓度大于0.50 mg/L时,AsA、DHA、总量Vc、AsA/DHA比值与对照相比均显著降低;在AsA循环中的抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)和单脱氢抗坏血酸还原酶(MDHAR)活性变化趋势相似,均随着处理浓度增加呈现出先增加后降低的趋势,其中以0.50 mg/L处理下酶活性最高,而抗坏血酸氧化酶(AAO)活性则随着处理浓度的增加而逐渐降低,以0.25 mg/L处理效果最好;在还原型谷胱甘肽(GSH)循环中,当处理浓度小于2.50 mg/L时,氧化型谷胱甘肽(GSSG)含量随着处理浓度的增加与对照相比稍有下降,但差异不显著,GSH含量与谷胱甘肽还原酶(GR)活性变化趋势相似,随着处理浓度增加呈现出先增加而后逐渐降低,其中以0.50 mg/L处理下效果最好。说明合理利用水葫芦中化感物质,有助于植株的生长,同时增加了体内AsA-GSH代谢循环,提高了青菜的抗氧化防御能力。

Effects of N-phenyl-2-naphthylamine on the growth and AsA-GSH circulation metabolism in Chinese cabbage were studied in the paper.The results showed that the different phenomena were found in the shoot height and biomass when the Chinese cabbages were treated by the different N-phenyl-2-naphthylamine concentrations.Especially,the treatment of 0.50 mg/L had the best effect.The shoot height and fresh weight significantly increased compared with the control in the different sampling periods.However,when the concentration of N-phenyl-2-naphthylamine was more 0.50 mg/L,the shoot height and biomass remarkably decreased with the increase of N-phenyl-2-naphthylamine concentration.The contents of reduced ascorbate（AsA）,dehydroascorbate（DHA）,total Vc and ratio of AsA/DHA had the same trends.In the treatments of 0.50 mg/L,contents of AsA,DHA,total Vc and ratio of AsA/DHA significantly increased compared with the control.When the concentration of N-phenyl-2-naphthylamine was more 0.50 mg/L,the contents of total Vc,AsA,DHA and ratio of AsA/DHA remarkably decreased with the increase of N-phenyl-2-naphthylamine concentration.In the AsA cycle,the activities of ascorbic acid peroxidase（APX）,dehydroascorbate reductase（DHAR）and monodehydroascorbate reductase（MDHAR） had the same change trends.All the activities showed the increase firstly and then showed the decrease with the increase of N-phenyl-2-naphthylamine concentration,especially,in the treatment of 0.50 mg/L,whereas the ascorbate oxidase（AAO） presented the gradually decrease trend with the increase of N-phenyl-2-naphthylamine concentration.The treatment of 0.25 mg/L had the best effect about the AAO activity.The content of oxidized glutathione（GSSG） showed a little decrease compared with the control in the GSH cycle when the concentration was lower than 2.50 mg/L.It showed no difference compared with the control.But the reduced glutathione（GSH） content and the glutathione reductase（GR） appeared the increase firstly and then the decrease with the increase of N-phenyl-2-naphthylamine concentration.The treatment of 0.50 mg/L had the best effect.All the results suggested that to reasonable use the allelochemicals in Water hyacinth could promote the growth,enhance AsA-GSH circulation metabolism and maintain higher antioxidative stress capacity in the Chinese cabbage.