期刊文献+

丹参酮ⅡA对压力负荷增加大鼠心肌纤维化的改善作用 被引量:12

Improvement of Tanshinone ⅡA on Myocardial Fibrosis in Rats with Pressure Overload
下载PDF
导出
摘要 目的:观察丹参酮ⅡA(TanⅡA)对压力负荷增加大鼠心肌纤维化的改善作用。方法:60只SD大鼠随机分为假手术组(Sham组,n=8)和手术组(n=52),手术组均行腹主动脉缩窄术制备压力负荷增加心肌纤维化模型,术后4周,存活的32只成模大鼠中8只留作模型组(Model组),余24只分为TanⅡA低剂量组(L-TanⅡA组,10mg/Kg/天)、TanⅡA高剂量组(H-TanⅡA组,20mg/Kg/天)及阳性对照药物卡托普利组(Captopril组,100mg/Kg/天),每组8例。给药4周后,检测5组大鼠心肌肥厚指数和心肌组织形态、心肌羟脯氨酸(HYP)含量、心肌组织Rho相关卷曲螺旋蛋白激酶1(ROCK1)、转化生长因子-β1(TGF-β1)和核转录因子-κBp65(NF-κBp65)蛋白含量。结果:与Sham组比较,Model组大鼠的心肌肥厚指数、心肌HYP含量及心肌组织中ROCK1、TGF-β1和NF-κBp65蛋白含量均显著升高(P均<0.01),组织病理学改变明显;与Model组比较,L-TanⅡA和H-TanⅡA组心肌肥厚指数、心肌HYP含量及心肌组织中ROCK1、TGF-β1和NF-κBp65蛋白含量均降低(P<0.05或P<0.01),组织病理学改变明显。结论:TanⅡA能改善压力负荷增加大鼠心肌纤维化,此作用可能与其抑制ROCK1表达,下调TGF-β1和NF-κBp65水平有关。 Objective: To observe the improvement of tanshinone ⅡA (TanⅡA) on myocardial fibrosis in rats with pressure overload. Method: 60 Sprague-Dawley (SD) rats were randomly divided into sham operation group (sham group,n=8) and surgical group (n=52). The rats of surgical group induced by the abdominal aorta constriction preparation. 32 rats of surgical group survived and were randomly divided into four groups (n=8):model group, TanⅡA low dose group (L-TanⅡA group), TanⅡA high dose group (H-TanⅡA group) and positive captopril group (captopril group). After 4 weeks, heart mass indexes were estimated, in concurrent evaluation of myocardial histology, as well as myocardial hydroxyproline content by ELISA, and immunohistochemistry staining for myocardial ROCK1, transforming growth factor-beta1 (TGF-β1) and nuclear transcription factor-kappa B p65 (NF-κB p65) protein content. Results: Compared with those in sham group, heart mass indexes, myocardial hydroxyproline content and myocardial ROCK1, TGF-β1 and NF-κB p65 content were increased in the model group (P<0.01); Compared with those in model group, heart mass indexes, myocardial hydroxyproline content and myocardial ROCK1, TGF-β1 and NF-κB p65 content were decreased in the L-TanⅡA group (P<0.05 or P<0.01), while these indexes were decreased significantly in the H-Tan Ⅱ A group (P<0.01). Conclusion: TanⅡA can improve myocardial fibrosis in rats with pressure overload and this effect may be related to inhibition of ROCK1 expression, downregulation of TGF-β1 and NF-κB p65 expression.
出处 《微循环学杂志》 2013年第2期34-36,39,F0003,F0004,I0001,I0002,共8页 Chinese Journal of Microcirculation
基金 南京总医院科研基金面上课题(2010Q027)
关键词 ROCK1 压力负荷 心肌纤维化 转化生长因子-Β1 核因子-ΚB P65 丹参酮ⅡA 大鼠 ROCK1 Pressure overload Myocardial fibrosis TGF-β1 NF-κB p65 TanshinoneⅡA Rat
  • 相关文献

参考文献3

二级参考文献27

  • 1陈运禄,黄文军,刘群.丹参对老年难治性心力衰竭患者氧化/抗氧化系统的作用[J].实用临床医学(江西),2004,5(4):15-17. 被引量:1
  • 2修春英,蔡辉.溶血磷脂酸与缺血性心脏病[J].医学研究生学报,2006,19(2):172-174. 被引量:3
  • 3Brilla CG, Zhou G, Masubam L, et al. Collagen metabolis in cultural a-dult rat cardiac fibroblasts: Rwsponse to angiotensin II and ,dldosterone [ J ]. Mol Cell Cardiol, 1994,26 : 809 - 820.
  • 4Brilla CG. Aldosterone and myocardial fibrosis in heart failure [ J ]. Herz,2000,25 (3) :299 -306.
  • 5Vare N,Iraburu MJ,Varela,et al. Chronic AT( 1 ) blockade stimulates extraeellular collagen type I degradation and reverses myocardial fibresis in spontaneously hypertensive rats [ J ]. Hypertension, 2000,35 (6) :1197 - 1202.
  • 6Brown RD,Ambler SK,Mitchell MD,et al.The cardiac fibroblast:therapeutic target in myocardial remodeling and failure[J].Annu Rev Pharmacol Toxicol,2005,45(6):657-687.
  • 7Sengupta S,Wang Z,Tipps R,et al.Biology of LPA in health and disease[J].Semin Cell Dev Biol,2004,15(5):503-512.
  • 8Karliner JS,Honbo N,Summers K,et al.The lysophospholipids sphingosine-1-phosphate and lysophosphatidic acid enhance survival during hypoxia in neonatal rat cardiac myocytes[J].J Mol Cell Cardiol,2001,33(9):1713-1717.
  • 9Lijnen PJ,Petrov VV,Fagard RH.Induction of cardiac fibrosis by transforming growth factor-beta(1)[J].Mol Genet Metab,2000,71(1-2):418-435.
  • 10Rosenkranz S.TGF-beta1 and angiotensin networking in cardiac remodeling[J].Cardiovasc Res,2004,63(3):423-432.

共引文献33

同被引文献200

引证文献12

二级引证文献77

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部