太行菊不同器官中绿原酸和4种黄酮类物质含量研究

Determination of Chlorogenic acid and Four flavonoids in Different Organs of Opisthopappus taihangensis(Ling)Shih

为深入研究我国特有植物太行菊,采用高效液相色谱法(HPLC),以ZORBAX Eclipse XDB-C18(4.6×150 mm,5μm)为分析柱,甲醇-0.01%磷酸水溶液梯度洗脱,流速1.0 mL/min,检测波长254 nm,柱温25℃;对太行菊和传统药用野菊不同器官的绿原酸、芦丁、槲皮素、木犀草素、芹菜素含量进行测定。此外,以芦丁为对照品,采用硝酸铝络合紫外分光光度法,以没食子酸为对照品,采用福林酚法,分别对太行菊和野菊不同器官中总黄酮和总多酚的含量进行研究。结果表明,基于上述HPLC检测方法,五种化合物的质量浓度在5.94～178.2、4.36～130.8、8.96～268.8、4.08～122.4、2.98～25.33 mg/L范围内与峰面积线性关系良好,相关系数r均大于0.9993,方法的精密度、重复性、稳定性及平均加样回收率试验结果均满足分析要求。太行菊叶中五种化合物的总含量高于其花、茎和野菊对应器官,与太行菊和野菊不同器官中总黄酮和总多酚含量分析结果一致。因此,太行菊整个植株,尤其是叶和花的开发利用潜力显著。

In this study,the content of chlorogenic acid,rutin,quercetin,luteolin and apigenin in Opisthopappus taihangensis（taihangensis）and Chrysanthemum indicum（indicum）were quantitatively determined by HPLC.ZORBAX Eclipse XDB-C18 column（4.6×150 mm,5 μm）was used for chromatographic separation with mobile phases consisted of methanol-0.01% phosphoric acid-water with gradient elution at a flow rate of 1.0 mL/min.The detection wavelength was set at 254 nm and column temperature was 25 ℃.Moreover,the total phenolic content was determined by Folin-Ciocalteu method using gallic acid as the standard.The total flavonoid content was analyzed by aluminum nitrate colorimetric method using rutin as the standard.Using the above HPLC method,the linear relationship between peak area and concentration for chlorogenic acid,rutin,quercetin,luteolin and apigenin in the ranges of 5.94~178.2,4.36~130.8,8.96~268.8,4.08~122.4,and 2.98~25.33 mg/L was good,with the correlation coefficients（r）greater than 0.9993.The performance characteristics of precision,reproducibility,stability and recovery of the developed HPLC method fulfilled the analysis requirements.The total content of five compounds in leaves of taihangensis was higher than those in their flowers and stems,and also higher than the contents in corresponding organs of indicum.These results were consistent with the result of total flavonoids and polyphenols content analysis in taihangensis and indicum.Therefore,huge utilization potential was expected from the whole plant of taihangensis,especially their leaves and flowers.