双T-DNA反式串联植物表达载体构建与验证

Construction and Verification of Trans-linked Double T-DNA Plant Expression Vector

通过对双T-DNA串联结构的改变,构建2个双T-DNA反式串联的植物表达载体p1300-DMAR-LF-GCMSAGS和p1300-DMAR-LF-GLCMSAGS。经过转化水稻后验证,结果表明:能够获得无选择标记基因的转基因植株;与顺式串联植物表达载体比较,反式串联的植物表达载体成功地将非连锁共整合的频率分别由47.37%和45.83%提高到了55.81%和51.28%,提高无选择标记基因转基因植株的筛选效率。

We constructed two trans-linked double T-DNA plant expression vectors by modification of the structure of the double T-DNA,p1300-DMAR-LF-GCMSAGS and p1300-DMAR-LF-GLCMSAGS.They were verified by transgenic rice plants.The results indicated that the selectable marker-free transgenic plants could be obtained.The frequencies of unlinked co-transformants transformed by the trans-linked double T-DNA plant expression vectors were increased from 47.37% and 45.83% to 55.81% and 51.28% compared to those transformed by the cis-linked double T-DNA plant expression vectors,indicated that the efficiency on the selectable marker-free transgenic plant was improved.