夏枯草内生菌及根际芽胞杆菌种群结构的研究

Study of the Population Structure of Bacillus Species Isolated from Medicinal Plant Selfheal and Its Rhizosphere Soil

芽胞杆菌分布广泛,主要分布于土壤、动植物体表。为了解采自武夷山的夏枯草Prunella vulgaris植株及其根际土壤芽胞杆菌的多样性,以及药用植物内生芽胞杆菌的生物多样性,采用稀释涂布平板法从夏枯草植株及其根际土壤样品中分离到芽胞杆菌菌株27株,并采用16SrDNA序列测定法对27株芽胞杆菌进行鉴定。通过菌落形态特征观察及16SrDNA序列测定,将27株芽胞杆菌归入14个种,其中阿氏芽胞杆菌5株、蜡状芽胞杆菌4株、炭疽芽胞杆菌4株、苏云金芽胞杆菌2株、甲基营养型芽胞杆菌2株、嗜气芽胞杆菌1株、地衣芽胞杆菌1株、假蕈状芽胞杆菌1株、简单芽胞杆菌1株、短短芽胞杆菌1株、球形赖氨酸芽胞杆菌1株、解木糖赖氨酸芽胞杆菌1株、嗜几丁质类芽胞杆菌1株,另外2株为芽胞杆菌疑似新种。研究表明,夏枯草植株及其根际土壤中存在着丰富的芽胞杆菌种类,丰富了药用植物内生芽胞杆菌资源的研究。

Bacillus is widespread around the world and mainly found in the soil and on the surface of plants and animals.We obtained 27 Bacillus isolates from selfheal and its rhizosphere soil using dilution plating technique in Wuyi mountain and then identified these strains by 16S rDNA sequencing to know the diversity of Bacillus inside the selfheal and its rhizosphere soil and enrich Bacillus distribution.Our results showed that 27 strains were classified into 14 Bacillus species and two strains may be new Bacillus species,suggesting that there was a diversity of Bacillus species in selfheal and its rhizosphere soil,and it enriched the study of the endogenous Bacillus in the medicinal plant.