豌豆抗白粉病资源筛选及分子鉴定

Screening and Molecular Identification of Resistance to Powdery Mildew in Pea Germplasm

由豌豆白粉菌引起的白粉病是豌豆生产上的重要病害,利用抗病品种是防治该病害最经济有效的方法。本研究在控制条件下苗期接种鉴定了396份豌豆资源对2个不同地理来源的豌豆白粉病菌分离物EPBJ和EPYN的抗性,用4个与豌豆抗白粉病基因er1连锁的SCAR标记对66份免疫或抗病资源进行标记基因型鉴定。结果表明,在鉴定的396份资源中,有101份资源表现免疫或抗病,其中对分离物EPBJ和EPYN免疫的资源分别为59份(14.9%)和60份(15.2%),对2个分离物均免疫的资源有54份(13.6%);在鉴定的82份中国资源中,有8份对2个分离物均表现免疫。分子标记将66份免疫或抗病资源鉴定为13个标记基因型,同一地理来源的抗性资源分属不同的标记基因型,其中8份来自中国云南的抗性资源分属7个标记基因型。研究表明,中国存在有效的豌豆白粉病抗源,抗性资源具有丰富的遗传多样性。

Pea powdery mildew, caused by Erysiphe pisi, is one of the most important diseases in pea production. The utilization of resistant pea cultivars is the most economical and effective method for controlling the disease. In this study, 396 pea accessions were evaluated for resistance to two powdery mildew isolates from different geographical origins in seedling stage under control- ling condition, and four SCAR markers closely linked to pea powdery mildew resistance gene erl were used to genotype acces- sions with immunity or resistance. The results showed that 101 accessions were immune or resistant to powdery mildew; 59 （14.9%） and 60 （15.2%） accessions were immune to the Beijing isolate and Yunnan isolate, respectively, 54 （13.6%） accessions were immune to both the Beijing isolate and Yunnan isolate. In 82 accessions from China, only eight were immune to both of the two isolates. Thirteen marker genotypes were identified in 66 selected accessions with immunity or resistance through molecular genotyping, and the accessions from the same geographical origin contained different marker genotypes. Eight accessions from Yunnan Province of China shared seven marker genotypes. These results indicated that pea germplasm with resistance to pea pow- dery mildew has effective and extensive genetic diversity in China.