miRNA-142-5p在特应性皮炎中的表达及其靶基因预测

Expression of miRNA-142-5P in atopic dermatitis and its target gene prediction

目的检测特应性皮炎(AD)患者外周血单个核细胞(PBMC)中miRNA-142-5p的表达情况,并对其进行潜在靶基因预测。方法选择2011年10月至2012年2月该院门诊皮肤科AD患者8例为观察组,与观察组年龄和性别相匹配的健康体检者6例为对照组。提取分离所有受试者PBMC中的miRNA,采用实时荧光定量PCR法,检测miRNA-142-5p的表达水平,运用miRanda、PicTar和TergetScan等靶基因预测分析软件等生物信息学方法,筛选其潜在靶基因。结果与对照组比较,miRNA-142-5p在观察组患者PBMC中的表达水平上调(P<0.05),预测到交集靶基因有39个,涉及到信号转导、细胞凋亡、细胞分化等。结论 miRNA-142-5p在AD患者PBMC中高表达,可能通过调控其下游靶基因在AD发病中起着重要作用。

Objective To detect the expression levels of miRNA-142-5p in peripheral blood mononuclear cell （PBMC） from the patients with atopic dermatitis （AD） and to predict its latent target genes. Methods 8 cases of AD treated in the dermatological outpatient department of this hospital from October 2011 to February 2012 were selected as the observation group and 6 age- matched and sex-matched individuals with physical examination as the control group. PBMC were isolated, rniRNAs in PBMC were extracted and separated by miRNA extraction and separation kit. The expression levels of miRNA-142-5p in PBMC were detected by using real-time quantitative PCR. Six databases including miRanda,PicTar and Targetscan were adopted for predicting the tar- gets of selected miRNAs. Results Compared with the control group, the relative expression levels of miRNA-142-5p in PBMC of AD patients were up-regulated （P〈0.05）. A total of 39 target genes for miRNA-142-5p were shared between these five databases, which might be involved in signal transduction,apoptotic process and cell differentiation. Conclusion miRNA-142-5p is up-regula ted in the PBMC of AD patients,which may play an important role in the pathogenesis of AD by regulating the downstream target gene.