妊娠母体不同阶段花生四烯酸乙醇胺水平变化分析被引量：2

下载PDF

导出

摘要目的检测妊娠母体妊娠过程中外周血及产前母体血浆中内源性大麻受体(CBR)的内源性配体花生四烯酸乙醇胺(AEA)水平的变化,并探讨其变化的临床意义。方法选择2010年10月至2011年3月在重庆医科大学附属第一医院妇产科产前检查和住院分娩的孕妇200例,根据妊娠时间不同分为孕早期(5～12周),孕中期(15～28周),孕晚期(36～39周)及产前4个组,每组50例。选择10名女性未孕的健康体检者为对照组。采集各组对象的静脉血,应用高效液相色谱法检测各血样标本中内源性大麻素AEA水平的变化,并对检测结果进行比较。结果孕早期开始到孕晚期,妊娠母体血浆中AEA水平具有一定降低趋势,孕中期及孕晚期母体血浆中AEA水平显著低于对照组女性静脉血浆中AEA,差异有统计学意义(P<0.01);临产孕妇母体血浆中AEA水平发生急剧升高,即刻孕妇静脉血浆中AEA水平增加到(18.64±3.95)ng/L,并显著高于妊娠母体孕中、晚期血浆中AEA水平(P<0.01)。结论妊娠过程中AEA水平发生显著变化,对于生殖过程可能意味着体内某种生理过程的改变;产前母体血浆中AEA水平发生了较大改变,在分娩期间AEA水平增高可能发挥了镇痛、舒张子宫平滑肌助产等生理作用。

作者封玉玲周海英李家莉苗加伟李晶周敏

机构地区重庆三峡医药高等专科学校病理教研室重庆三峡中心医院重庆三峡医药高等专科学校药理教研室重庆医科大学附属第一医院妇产科

出处《重庆医学》 CAS CSCD 北大核心 2013年第15期1758-1759,共2页 Chongqing medicine

基金重庆市教委科学技术研究项目(KJ111802)

关键词孕妇血浆花生四烯酸乙醇胺色谱法超临界液相

分类号 R714.1 [医药卫生—妇产科学]

引文网络
相关文献

参考文献10

1Ameri A. The effects of cannabinoids on the brain[J].Prog Neurobioh 1999,58(4) : 315-348.
2Dennedy MC,Friel AM, Houlihan DD,et al. Cannabinoidsand the human uterus during pregnancy[J]. Obstet Gyne-col,2004,190(l):2-9.
3李晶,董志,乐乐乐.脑缺血/再灌注对大鼠脑内花生四烯酸乙醇胺浓度的影响及其机理初步研究[J].激光杂志,2010,31(3):93-93. 被引量：5
4Van Sickle MD,Duncan M, Kingsley PJ,et al. Identificationand functional characterization of brainstem cannabinoid CB2receptors[J]. Science,2005,310(5746) :329-332.
5Park B,Gibbons HM,Mitchell MD,et al. Identification ofthe CB1 cannabinoid receptor and fatty acid amide hydro-lase (FAAH) in the human placenta[J].Placenta,2003,24(10):990-995.
6Paria BC? Song H, Wang X, et al. Dysregulated cannabi-noid signaling disrupts uterine receptivity for embryo im-plantation[J]. Biol Chem,2001,276(23) :20523-20528.
7Sun X,Dey SK. Cannabinoid/endocannabinoid signalingimpact on early pregnancy events [ J]. Curr Top BehavNeurosci,2009,l(l) :255-273.
8Wang H, Xie H, Dey SK. Loss of cannabinoid receptor CB1induces preterm birth[J].PLoS One,2008?3(10) :e3320.
9Sordelli MS, Beltrame JS,Burdet J,et al. The effect of anan-damide on uterine nitric oxide synthase activity dependson the presence of the blastocyst[J]. PLoS One,2011,6(4):el8368.
10Mitchell MD,Sato TA , Wang A,et al. Cannabinoids stimulateprostaglandin production by human gestational tissuesthrough a tissue- and CBl-receptor'specific mechanism[J].Am J Physiol Endocrinol Metab,2008,294(2) :E352-356.

二级参考文献7

1Longa E Z, Wenstein P R, Carlson S. Reversible meddle cerebral artery occlusion without craniectomy in rat[J].Stroke, 1989,20(1) :84 - 91.
2Arai Y, Fukushima T, Shirao M, Yang X J. Sensitive dettermination of anandamide in rat brain utilizing a coupled - column HPLC with fluorimetric detection[J]. Biomed. Chromatogr,2000,14:118 - 124.
3Keizo WAKU. Endogenous Cannabinoid Receptro Ligands- Anandamicde and 2 - Arachidonoylglycerol[J].The Pharmaceutical Society of Japan, 2006,126(2) :67 - 81.
4Zhang M, Martin BR, Adler MW, Razdan RK, Ganea D, Tuma RF. Modulation of the balance between cannabinoid CB ( 1 ) and CB (2) receptor activation during cerebral ischemie/reperfusian injury [ J ]. Neuroscience, 2008,152(3) :753 - 760.
5Hillard CJ. Role of cannabinoids and endocannabinoids in cerebral ischemia] . Curt Pharm Des,2008,14(23) :2347 - 2361.
6Makriyannis A, Mechoulam R, Piomelli D. Therapeutic opportunities through raodulation of the endocannabinoid system[J].Neurophannacology,2005,48(8) : 1068 - 1071.
7Igor Grant, B. Rael. CahnbCarmabis and endocannabinoid modulators: Therapeutic promises and challenges[J]. Clin Neurosci Res,2005,5(2 - 4) : 185 - 199.