尼卡地平和表皮生长因子对胶质瘤细胞生长的影响(英文)

Nicardipine inhibits growth stimulating effect of epidermal growth factor on human glioblastoma cells

观察钙通道拮抗剂尼卡地平(nicardipine)和表皮生长因子(epidermalgrowthfactor ,EGF)对恶性胶质瘤细胞系U251MG生长的作用。方法 :在含10%小牛血清 (fetalbovineserum,FBS)和无血清培养基中 ,予不同浓度的EGF、尼卡地平以及尼卡地平和EGF(10ng/ml)干预 ,应用MTT比色法观察它们的细胞生长效应。结果 :在无血清培养基中 ,EGF具有强大的浓度依赖性细胞生长刺激作用 ,10ng/ml接近最大效应。10 %小牛血清的促生长效应接近无血清条件下EGF的作用。尼卡地平能抑制U251MG细胞的生长,在有血清培养基中作用更明显 ;且尼卡地平能完全阻断EGF的生长刺激效应 ,U251MG细胞对尼卡地平和EGF(10ng/ml)的生长反应与对单一尼卡地平的相仿。结论 :在无血清条件下 ,EGF对U251MG细胞具有较强的促生长作用 ;血清的存在可掩盖EGF的作用 ,EGF与血清并不表现出明显的协同效应。尼卡地平能抑制胶质瘤细胞的增殖 ,并能完全阻断EGF的生长刺激效应 。

Objective: To observe and analyze the effects of epidermal growth factor (EGF) and the calcium channel antagonist nicardipine on the growth of U251MG, a human malignant glioma cell line, which possesses high affinity EGF receptors Methods:The growth effects of EGF and nicardipine on U251MG cultured in serum free and serum supplemented (10%fetal bovine serum, FBS) medium respectively were observed by using MTT colorimeritric analysis Results: ①EGF significantly enhanced the growth of U251MG cells in a concentration dependent manner in serum free medium The maximal effect was seen at 20 ng/ml,which was approximated to those obtained in 10%FBS ②Nicardipine decreased U251MG cell proliferation, especially in serum supplemented medium, and completely blocked the growth stimulating effects of EGF The combined effects of EGF (10ng/ml) and nicardipine equaled to those of nicardipine alone Conclusions: Nicardipine can suppress the growth of U251MG cells and completely block the growth stimulating effects of EGF,which suggests this drug could be a potential adjuvent therapeutic agent for human malignant gliomas