黄芩中酚性甙类对小鼠颌下腺放射损伤防护的研究

Protective effect of phenolic hydroxyl from scutellaria baicalensisgeorgi on radiation injury in submandibular glands of mice

探索黄芩中酚性甙类对小鼠颌下腺放射损伤的防护作用。方法 :黄芩水提液酸化保温 (80℃ )50min。滤去析出的黄芩酮类 (FSBG) ,水溶液用经典分离得主要有效成分为酚性甙类沉淀物 (I)。用60Co3 5Gy一次全身照射NIH雌性小鼠 ,照前35～45min每鼠腹腔注射(I)1 2g/Kg ,并设单纯放射组和空白对照组。分别于放射后13～17d解剖小鼠取其颌下腺称重并作电镜切片比较各组差异。实验重复3次。结果 :每次实验结果均显示照射前给小鼠腹腔注射 (I)对放射引起的颌下腺损伤有显著防护作用 ,表现为颌下腺重量和单放组有显著差异和电镜下超微结构的差异。结论 :黄芩中酚性甙类对小鼠颌下腺的放射损伤有防护作用 。

Objective:To investigate protective effect of phenolic hydroxyl on extructed from of scutellaria baicalensis georgi(SBG) radiation injury of submandibular glands in mice Methods: the heat extracted liquid from SBG was acidified and kept hot for 50 minutes at 80℃ Then the flavonoids of SBG (FSBG) were precipitated and removed by filter A precipitate (I) possessing effective component glycoside of SBG(GS GB) was obtained by classical separation process from the filtered solution Intraperitoneal injection (ip) of (I) 1 2g/kgin NIH female mice 35～45 minutes before 3 5 Gy of whole body 60C0 irradiationwas conducted At the same time, irradiation control group and free control group were assigned respectively The mice were sacrificed 13～17 days after irradiation Their submandibular glands were weighed and prepared for electron microscope examination The difference among three groups were compared The experiment was repeated three times Results: Every experiment showed that the intraperitoneal injection of (I) 1 2 g/kg in mice have significant protective effect on radiation injury to submandibular glands The difference of weight of submandibular glands between treatment group and irradiation control group was remarkable The ultrastructure changes of these two groups were also significantly different Conclusion: 1 Glycoside of SBG may have protective effect on radiation injury of submandibular glands in mice 2 This study suggests that GS BGcould be used in clinic as a protector of radiation injury for parotid gland