低密度脂蛋白胆固醇的直接测定法

The method of direct assay for low density lipoprotein cholesterol

目的 建立直接测定血清低密度脂蛋白胆固醇 (LDL C)的方法。方法 基于选择性水解法的原理 ,通过对多聚体和表面活性剂的筛选和测定条件的优化实验确定了方法。结果 本法反应达终点的时间为 180s ,线性达 11 7mmol/L ,批内CV <1 6 4% ,日间CV <2 6 5 % ,总CV <3 38% ,回收率平均为 99 7%。和日本一化试剂比较 :Y =0 94X + 0 0 32 ,r =0 975 ,n =5 2。本法的特异性好 ,加入LDL C达 3 9mmol/L ,VLDL Trig达 19 5mmol/L ,抗坏血酸 <2 84mmol/L、血红蛋白<5g/L和胆红素 <340 μmol/L时无显著干扰。 结论 本法的总误差 <7 40 % ,达到NCEP提出的 <12 %的分析目标 ,标本无需预处理 。

Objective A method of direct assay for low density lipoprotein cholesterol(LDL*.C)was developed.Method Based on the principle of the enzymatic seleective hydrolysis methodology,to select the kinds and concentrations of polyanion and detergent,and the conditions of analysis were estabilished by optimization experiment.Results The end of reactive time was 180 sec.The linearity of this method was up to 11 7 mmol/L,within-run CV was 1 62%,between-run CV was 2 65%,and total CV was 3 21% The recovery was 99 7% The new assay( Y )was compared to DAIICHI reagent( X ): Y=0 94X+0 032,r=0 97,n=52 No interference were showed when addition of isolated HDL fraction(HDL-C up to 3 9 mmol/L)and VLDL fraction(VLDL-Trig up to 19 5 mmol/L),ascorbic acid<2 84 mmol/L,Hb<5 g/L and bilirubin<340 μmol/L to pooled serum,represently.Conclusion The total error of this method was <7 40% to achieved NCEP total error goal of <12% for LDL-C measurement.No pretreatment of the sample was required,and suitable for automated.