乙酸预浸对玉米秸秆蒸汽爆破预处理的影响

The Effect of Acetic Acid Impregnation on Steam Pretreatment of Corn Stover

对经乙酸预浸汽爆预处理的玉米秸秆进行了组分含量和抑制物分析,并研究了玉米秸秆预处理后的酶水解性和同步糖化发酵。与未经乙酸预浸相比,乙酸预浸玉米秸秆能在相对低温下进行汽爆预处理,在提高半纤维素水解程度的同时,并不会明显增加糠醛等发酵抑制物。酶水解实验表明,玉米秸秆经乙酸预浸,再以200℃进行汽爆后的酶水解效果较好,每克原料可获得284 mg葡萄糖,提高了10.2%,为理论值的76.8%;乙酸预浸玉米秸秆经过96 h同步糖化发酵,获得了22.5 g/L的乙醇浓度,为理论值的72%;相比未经乙酸预浸的玉米秸秆,提高了11.9个百分点。

In this study, compositions and inhibitors of steam pretreated corn stover with acetic acid impregnation were analyzed. Enzymatic hydrolysis and simultaneous saccharification fermentation of corn stover pretreated at corresponding situation were investigated. The results show that acetic acid impregnated corn stover can be steam exploded at relatively low temperature that is profitable to increase the hydrolysis rate of hemicellulose and the recovery of xylose. The enzymatic hydrolysis experiment showed that when corn stover was impregnated with 1.0 % HAc at 200℃ for l0 min, a glucose yield of 284 mg per 100 g corn stover, corresponding to 76.8 % of theoretical maximum, was obtained. After 96 h SSF,the end ethanol concentration is 22.5 g/L, corresponding to 72 % of the theoretical value. It is 11.9 percent point higher than that without acetic acid impregnation.