毛细管电泳测定当归饮片中阿魏酸含量

Determination of Ferulic Acid in the Root of Angelica sinensis

研究了用反向毛细管电泳分离测定当归中阿魏酸含量的条件。当归饮片用热水提取 ,电泳缓冲液为 Tris-H3BO3(2 0∶ 80 mm ol/ L) ,并加入 0 .8mm ol/ L 的 CTAB作为电渗流改向剂 ,分离电压为 - 10 .0 k V,用安培检测器检测。分析结果每克当归饮片含阿魏酸 0 .415 mg,RSD2 .3 % ,回收率为 97.1%

Experimental conditions for the separation and determination of ferulic acid in the root of Angelica sinensis (Oliv ) Diels by capillary electrophoresis were studied Sliced A sinensis was extracted with hot water, and the ferulic acid in the extract was separated by capillary electrophoresis and detected by amperometric detector Buffer consisted of 20 mmol/L of tris (hydroxymethyl) aminomethane and 80 mmol/L of boric acid 0 8 mmol/L of cetyltrimethyl ammonium bromide was added as the counter electroosmotic flow agent Separation voltage was -10 0 kV Result of the study showed that 1 g of the dry herb contains 0 415 mg of ferulic acid (RSD=2 3%, γ=97 1%)