小波变换-比值导数光谱法同时测定脑清片中氨基比林和咖啡因的含量

SIMULTANEOUS DETERMINATION OF AMINOPHENAZONE AND CAFFEINE IN NAOQING TABLETS BY WAVELET TRANSFORM RATIO DERIVATIVE SPECTROPHOTOMETRY

目的 建立小波变换 比值导数光谱法 (WT RDS) ,考察其在多组分样品含量测定中去除高频噪音干扰的作用。方法 采用WT RDS同时测定脑清片中氨基比林和咖啡因的含量 ,以两组分样品的原始光谱除以其中某一组分的标准光谱后得到比值光谱 ,再以比值光谱对波长求导得到比值导数光谱。选取适当波长的振幅值测定 ,可消除一个组分的干扰而测定另一组分的含量 ;比较各实验数据小波变换前后处理的结果。结果 采用小波变换技术可消除测定组分的比值导数光谱中被“放大”的噪音的影响 ,实验数据的线性关系、精密度等得到改善。结论 WT RDS灵敏度高 ,抗干扰能力强 。

AIM To establish wavelet transform ratio derivative spectrophotometry (WT RDS) and test its application in removing high frequency noise for determination of multi component samples. METHODS Simultaneous determination of aminophenazone and caffeine in Naoqing tablets was achieved by using ratio derivative spectrophotometry. The ratio spectrum is obtained by dividing the amplitude of the UV absorption spectrum of the mixture of the two components by a standard UV spectrum of one of the components, and the subsequent derivation against wavelength results in the first derivative of the ratio spectrum. Once a positive wavelength with available amplitude value was chosen, the interference of one component can be removed and meanwhile the concentration of the other component was obtained. The processed date through and not through wavelet transform were compared. RESULTS By means of wavelet transformation the effect of the “enlarged” noise in the ratio derivative spectrum of a component can be eliminated. The linearity and precision of the data are improved. CONCLUSION The method WT RDS has the advantage of high sensitivity,stronger ability of removing interference and it gives satisfactory results.