PTPN11基因及幽门螺杆菌与广西柳州地区胃癌易感性关系的研究

Study of PTPN11 and helicobacter pylori with susceptibility to gastric cancer

目的探讨蛋白酪氨酸磷酸酶非受体型11(PTPN11)基因的多态性及幽门螺杆菌(Hp)感染与广西柳州地区人群胃癌易感性的关系。方法胃癌组(238例)及健康对照组(112例)均来自广西柳州地区。运用聚合酶链反应(PCR)及两步法聚合酶链反应(PCR-CTPP)技术对Hp的尿素酶B亚单位(UreB)基因及PTPN11基因第3内含子2460位点的单链构象多态性进行分析。结果胃癌组Hp阳性率高于健康对照组(59.7%vs 48.2%,P<0.05)。胃癌组等位基因A频率低于对照组,等位基因G频率高于对照组(P<0.05)。2组间PTPN11基因第3内含子2460位点各基因型(G/G型、G/A型和A/A型)频率差异无统计学意义(P>0.05)。与G/G型比较,A/A型和G/A型胃癌易感性在2组间差异无统计学意义(A/A型:OR=0.399,95%CI 0.097～1.641;G/A型:OR=0.642,95%CI 0.397～1.039);但A/A型和G/A型合并后再与G/G型比较,含A基因者胃癌易感性显著降低(OR=0.620,95%CI 0.388～0.992)。结论广西柳州地区的人群中PTPN11第3内含子2460位点携带A基因者胃癌易感性低,而该位点为G/G型及Hp感染者胃癌的易感性高。

Objective To investigate the protein tyrosine phosphatase 11 （PTPN11 ） gene polymorphism and Hp in- fection effect on susceptibility to gastric cancer in Liuzhou area of Guangxi. Methods The research object of gastric cancer group （ n =238） and control group（ n= 112） were derived from the Liuzhou area of Guangxi. Using the polymerase chain reaction （PCR） technique analysis of urease B subunit （UreB） gene Hp in two steps, using the polymerase chain reaction （PCR CTPP） technique of single strand conformation polymorphism analysis of position 2460 in intron 3 of the PTPNll. Re- sults The positive rate of Hp in gastric cancer group was higher than that of control group （59.7% vs 48.2%, P 〈 0.05 ）. Gastric cancer group and control group individuals of each genotype （ G/G, G/A, and A/A） at position 2460 in intron 3 of the PTPN11 distribution difference frequency was not significant （ P 〉 0.05 ）. As the group of high frequency distribution in gastric cancer group was higher than that of G allele of PTPN 11 （84r 5% vs. 78.1% ）, the control group and the distribution of allele frequency of A was lower than that （ 15.5% vs. 21.9% ）, there was significant difference between the two groups （P 〈 O. 05）. Compared with G/G type, A/A type and G/A type differences in susceptibility to gastric cancer was not signifi- cant between the two groups. （ A/A ： OR = 0. 399, 95% CI 0. 097 ~ 1. 641 ; G/A ： OR = 0. 642, 95% CI 0. 397 - 1. 039 ） ; The comparison of A/A and G/A combined with G/G, A genotype was significantly decreased the risk of stomach cancer （ OR = 0. 620, 95% CI 0.388 - 0.992）. Conclusion Carrying the A genotype in Liuzhou area of Guangxi individual at posi- tion 2460 in intron 3 of the PTPN11 for gastric cancer risk is significantly reduced, and the site for the association of G/G gene and Hp infection in gastric cancer is high.