大肠良恶性病变p53、PCNA的表达与S_(100)^+DC浸润的临床病理意义

Expression of p53, PCNA protein and infiltration of S_(100)^+DC in benign and malignant large intestinal lesions and their clinicopathological significance

目的 :探讨 p5 3、PCNA的表达和 S- 10 0阳性树状细胞 (S1 0 0 + DC)浸润在大肠肿瘤发生发展中的作用及意义。方法 :应用免疫组化 L SAB法检测 6 4例大肠腺癌、36例大肠腺瘤、32例大肠炎性组织 p5 3、PCNA的表达及S1 0 0 + DC浸润。结果 :p5 3在大肠腺癌、大肠腺瘤、大肠炎性组织的阳性表达率分别为 6 4.1% (4 1/6 4)、8.3% (3/36 )和 0 (0 /32 ) ,癌与非癌组织间有显著性差异 (P <0 .0 5 ) ;PCNA的阳性表达率在大肠腺癌、大肠腺瘤及大肠炎性组织中分别为 82 .8% (5 3/6 4)、5 2 .8% (19/36 )、18.8% (6 /32 ) ,癌与非癌组织间有显著性差异 (P<0 .0 5 )。 S1 0 0 + DC浸润率在大肠腺癌、大肠腺瘤及大肠炎性组织分别为 37.5 % (2 4/6 4)、6 1.1% (2 2 /36 )、6 2 .5 % (2 0 /32 ) ,癌与非癌组织间有显著性差异 (P<0 .0 5 )。 p5 3和 PNCA阳性表达率与组织学分组和临床分期有一定关系 ,而 S1 0 0 + DC则无明显相关。 结论 :p5 3和 PNCA的高表达是大肠癌恶变的分子标志 ,阳性程度越高 ,癌的分化程度越低 ,浸润性越强。

Objective:To study the expression of p53, PCNA and infiltration of S 100 \++DC in carcinogenesis and progression of large intestinal neoplasms and their significance. Methods: Expression of p53, PCNA and infiltration of S 100 \++DC were detected in 132 large intestinal lesions (including large intestinal carcinoma 64, large intestinal adenoma 36, large intestinal inflammation 32 by immunohistochemical( LSAB) technigue. Results: The positive frequency of p53 was 64.1% (41/64), 8.3%(3/36) and 0(0/32) in adenocarcinoma,adenoma and inflammatory tissues respectively; a significant difference between malignant and benign lesions was found (P<0.05). Expression of PCNA was 82.8%(53/64), 52.8%(19/36) and 18.8(6/32) in adenocacinoma, adenoma and inflammatory tissues respectively; a significant difference was found among them (P<0.05) . S 100 \++DC infiltrating rate was 37.5%(24/64), 61.1%(22/36) and 62.5%(20/32), in adenocarcinoma, adenoma and inflammatory tissues; a significant difference was found between malignant and benign lesions (P<0.05). The positive expression frequency of p53 and PCNA were related to histological grades and clinical stages in large intestinal adenocarcinoma but the infiltration of S 100 \++DC was not related to them. Conclusions: The expression of p53 and PCNA with high frequency could be identified as a molecular marker of malignant lesions reflected that immumocompetence of the host may be an important factor of carinogenesis in large intestine.