Lactobacillus casei-14对小鼠溃疡性结肠炎的预防作用

Preventive effects of Lactobacillus casei-14 on ulcerative colitis in mice

采用2.5%右旋葡聚糖硫酸钠（DSS）诱导小鼠溃疡性结肠炎模型,研究Lactobacillus casei-14（L.casei-14）对小鼠溃疡性结肠炎（UC）的预防作用,并探讨其可能的机制.结果表明：L.casei-14高剂量组小鼠结肠长度为（7.66±0.58）cm,与模型组（（6.73±0.42）cm）相比差异显著（P〈0.05）;中、高剂量组小鼠结肠炎疾病活动指数（DAI）分别为（4.22±0.97）和（4.17±1.33）,与模型组（8.00±2.12）相比差异极显著（P〈0.01）;组织病理学炎症评分结果显示,中、高剂量组小鼠炎症评分分别为（4.88±0.83）和（4.13±0.64）,极显著低于模型组（10.50±1.85）（P〈0.01）;中、高剂量组小鼠IL-1β、TNF-αmRNA表达水平均显著低于模型组,与正常对照组没有极显著性差异（P〈0.01）.说明L.casei-14对小鼠溃疡性结肠炎具有预防作用,其机制可能是通过抑制IL-1β和TNF-α炎症细胞因子的表达而减轻小鼠结肠黏膜的损伤,以达到预防UC的目的.

The model of UC was induced with 2.5% of dextran sulfate sodium（DSS） in mice,the preventive effects of Lactobacillus casei-14（L.casei-14） on ulcerative colitis（UC） were studied,and the possible mechanism was discussed.The results showed that compared with the model group（（6.73 ± 0.42）cm）,the colon length was significantly increased（P0.05） in L.casei-14 high-dose group（（7.66±0.58）cm）.DAI of medium-and high-dose groups were（4.22±0.97） and（4.17±1.33） respectively,and showed a contrasting significant reduction compared with the model group（8.00±2.12）（P0.01）;Histopathological score of medium-and high-dose groups were（4.88±0.83） and（4.13±0.64） respectively,and significantly reduced compared with the model group（10.5±1.85）（P0.01）.The expression levels of IL-1β,TNF-α mRNA in medium-and high-dose groups were significantly lower than that in the model control group.There was no remarkable difference compared with the normal group（P0.01）.L.casei-14 showed preventive effects on ulcerative colitis,and reduced the damage of colonic mucosa in mice.The mechanism was probably through inhibiting the expression of inflammation cytokines of IL-1β and TNF-α and to prevent or control UC.