温水处理对甘蔗白叶病植原体的脱除效果研究

The Effect of Hot-Water Treatment on Eliminating Sugarcane White Leaf Phytoplasma

甘蔗白叶病(SCWL)是一种易随种苗进行远距离传播的危险性检疫病害。近年从缅甸、菲律宾和法国等国引进的甘蔗品种/材料SCWL植原体阳性检出率高达78%,存在严重的引种安全隐患。本研究选择15个经巢式PCR检测确认带有SCWL植原体的引进甘蔗品种/材料进行温水(50±0.2)℃处理,采用巢式PCR检测SCWL植原体,研究温水处理对SCWL植原体的脱除效果。结果表明:温水处理2 h不能有效脱除SCWL植原体,脱除率仅为26.7%。因此,今后在加强与东南亚国家合作深入开展甘蔗品种/材料交换工作中,要重点加强对SCWL植原体检疫检测,一旦发现带有SCWL植原体的品种/材料必须及时进行销毁处理,避免SC-WL植原体随引进品种/材料侵入我国境内扩散蔓延,确保我国蔗糖产业的安全可持续发展。

Sugarcane white leaf disease （SCWL） is an important quarantine disease which is easy to be transmitted by seedling in a long range, and its positive rate has been up to the 78% in the sugar cane varieties/ materials introduced from Burma, Philippines, France and other countries in recent years, and has become a serious security risk in variety introductions. In this study, 15 introduced sugarcane varieties with SCWL phytoplasma confirmed by nested PCR were used as materials, the con trol effect of hotwater treatment at （50 ±0. 2） ℃ for 2 h on SCWL was studied, combined with nested PCR to detected SCWL phytoplasma. The results indicated that phytoplasma could not be eliminated effectively by hotwater treatment at （ 50 ± 0.2 ） ℃ for 2 h, the rate of phytoplasma removing only reached 26.7%. Therefore, in the work on promoting cooperation with Southeast Asian to carry out sugarcane varieties/materials exchanges, strengthened quarantine and detection of SCWL phytoplasma must be taken. Once found SCWL phytoplasma, the introduced varieties/materials must be destroyedpromptly to avoid the invading and spreading of SCWL phytoplasma through varieties/materials introduction and secure sustainable development of sugar industry in China.