分光光度法快速测定活性污泥浓度的方法研究

Rapid determination of activated sludge concentration with spectrophotometric method

污泥浓度作为城市污水处理厂设计和运行管理过程的一个重要参数,目前主要采用重量法测定,该方法在应用过程中最大的缺点是耗时耗能.本研究采用分光光度法在OD600处测定活性污泥的浓度,建立测定的方法,优化测定的预处理条件,并对测定结果进行了误差分析.结果表明,分光光度法测定活性污泥浓度的较佳范围为0.996～3.864g/L,较佳的稀释液和空白应为超纯水,预处理的最佳条件为功率110W时超声处理4min.总之,分光光度法具有节能、省时、误差小的特点,是一种简单实用的污泥浓度测定方法.

The suspended solid concentration in mixed liquid （MLSS） of activated sludge is an important parameter for designing and operating a wastewater treatment plant and is usually determined by gravimetric analysis, which is time-consuming and energy-consuming. This paper aimed to apply a procedure based on spectrophotometric analysis for rapid quantifying MLSS concentration of activated sludge at the wavelength of OD600. The pretreatment condition was optimized and measurement error was analyzed later after a detailed recipe was proposed. The results showed that the preferable range of activated sludge concentration ranged between 0. 9960-3. 864gMLSS/L. As for the diluent and blank in the spectrophotometric analysis, deionized water was favored. Meanwhile the optimal condition of ultrasonic pretreatment was under ll0W for 4 minutes. Compared with gravimetric method, spectrophotometrie analysis is a time-thrift, energythrift, low error method to determine the suspended solid concentration of activated sludge.