初夏一起腺病毒感染暴发性流行

An epidemic outbreak ofadenovirus infection earlythis summer

2000年5月中旬,某学校在2—3天内,相继有120多人出现高烧、周身酸痛,部分病人腹泻、扁桃腺肿大,但是血象不高,少有咳嗽,流涕等呼吸道症状,疑似病毒性感染,遂进行现场调查,采集部分病人和部分未发病者的静脉血、咽拭子和肛拭子送实验室检验。从咽拭子获得有脱落上皮细胞。

An epidemic outbreak of acute disease with symptoms of high fever and myalgia occurred in a school between Beijing and Tianjin. More than 120 students were abruptly involved within three days (17-19 June, 2000). Pateients had gastroenteric tract and/or upper respiratory tract symptoms characterized by diarrhea, tonsillitis, cough and rhinorrhea. A series of specimens of sera, throat swabs and anal swabs from patients and the healthy population at the same school were collected and sent to the laboratory for diagnostic test. The exfoliated cells from seven throat swabs were examined for adenovirus antigen by indrect immunofluorescence test. Three swabs were positive. One of the three swabs was further examined for adenovirus serotype identification by nested PCR and SSR ( sequencing of a serotype-specific region) . It was identifed as adenovirus serotype 5. Out of the 68 from patients, 52 (76.47%) manifested specific anti-adenovirus IgM by a commercial ELISA kit. Whereas, 37 of the 100 serum samples from the healthy control were also anti-adenovirus IgM positive (37%). It indicated that silent adenovirus infection occurred. The statistic analysis was performed by x2 test. The very signf-icant difference was obtained between these two populations. All the results above suggested that this epidemic outbreak was due to adenovirus infection.