结核杆菌(TB)PCR荧光诊断试剂盒的临床应用

Clinical application of PCR technique with gene－marked flourotagger probefor detecting mycobacterium tuberculosis

探讨半定量TB－PCR 荧光探针诊断试剂盒的临床应用价值.方法 以双盲法对115例结核病和59例非结核病人的痰标本进行厚涂片抗酸染色检查、结核菌培养、荧光探针 TB－PCR 检查对比.结果 三种方法检测的敏感性分别为32．2%、59．1%、71．3%,荧光探针TB－PCR 法的敏感性显著高于培养法和涂片法(P＜0．01和P＜0．05).特异性分别为93．2%、91．5%、94．9%,差异无显著性.结论 半定量TB－PCR荧光探针诊断试剂盒可应用于临床结核杆菌检测.

 Objective To evaluate the clinical application of semiquantitative PCR technique with gene－marked flourotagger probe GMFP for detecting mycobacterium tuberculosisTB． Method Thick smears of sputum from 115 patients with tuberculosis 59 patients without tuberculosis were detected by acid－fast stain culture and GMFP－PCR method and the results were compared． Results The sensitivity was 32．2% by smear 59．6% by culture and 71．3% by GMFP－PCR method respectively． The sensitivity of GMFP－PCR method was obviously higher than that of smearP＜0．01 and cultureP＜0．05． The specificity was 93．2% by smear 91．5% by culture and 94．9% by GMFP－PCR method respectively showing no significant difference． Conclusion The semiquantitative GMFP－TB－PCR method is an efficient way for the detection of TB． 