组合靶基因自动检测仪快速检测人乳头瘤病毒

Application of piezoelectric genosensor techniques for rapid detection of human papillomavirus

目的 探讨检测尖锐湿疣组织中人乳头瘤病毒 (HPV)的方法。方法 将基因芯片与压电传感器技术相结合 ,对复发型尖锐湿疣及相应的原发病例组织标本各 2 2份 ,利用HPV6、11、16、184种型特异寡核苷酸探针 ,进行HPV检测 ,并与常规的聚合酶链反应 (PCR)和斑点杂交方法比较。结果 用压电基因传感器芯片技术检测 ,原发型组织标本 2 2份全部为HPV阳性 ,其中HPV6 17份(77 3 % ) ,HPV113份 (13 6 % ) ,HPV16 2份 (9 1% ) ,HPV18未检出 ;复发型组织标本 2 2份中 ,2 1份阳性 ,其中HPV6检出 15份 (6 8 2 % ) ,HPV11检出 2份 (9 1% ) ,HPV16检出 4份 (18 2 % ) ,HPV18未检出 ,全部操作仅需 10min。用PCR方法检测结果全部阳性 ,PCR扩增的产物经斑点杂交分型 ,结果与其基本一致。结论压电基因传感器芯片技术用于检测HPV ,具有准确、快速、灵敏的优点。

Objective To establish a method for rapid detection and sub typing of human papilloma virus (HPV) in biomedical samples. Methods We utilized the piezoelectric genosensor technique, a combination of the piezoelectric biosensor and gene chips for HPV identification, in 22 cases of recurrent biopsy specimens and 22 cases of corresponding original biopsy specimens. The specimens were also analyzed by conventional PCR and Dot blot methods.Results In the 22 recurrent cases, 15 had HPV6 DNA, 2 contained HPV11 DNA, and 4 harbored HPV16 DNA. Only one case was negative. In the 22 original cases, all were positive: 17 harbored HPV6 DNA, 3 contained sequence homologous HPV11 DNA, and 2 harbored HPV16 DNA. No HPV18 DNA was detected in any specimen. When compared with the PCR and Dot blot analysis, the results were essentially the same except one case, which was shown to contain other sub typing of HPV.Conclusion Our results showed that piezoelectric genosensor technique is a rapid and specific method to analyze HPV.