泌尿生殖道嗜血杆菌的感染及临床意义

Haemophilus spp. in genitourinary infections: isolation and clinical significance

目的 了解嗜血杆菌在泌尿生殖道感染患者泌尿生殖道分泌物标本中的分离率及其生物型与临床意义。方法 选用嗜血杆菌专用培养基 (HAE)和APINH鉴定系统对 5 0 2 2份泌尿生殖道感染标本进行分离培养与鉴定。结果 分离出嗜血杆菌 198株 (3.9% ) ,其中副流感嗜血杆菌 139株、流感嗜血杆菌 5 4株、嗜沫 /副嗜沫嗜血杆菌 5株。进一步生化分型研究结果提示 :Ⅱ型副流感嗜血杆菌分离率最高 (2 9.3 % ) ,其次为Ⅲ型副流感嗜血杆菌 (2 4.8% )与Ⅳ型流感嗜血杆菌 (19.2 % )。嗜血杆菌阳性检出率在感染标本中以男性尿道分泌物为最高 (39.9% )。结论 嗜血杆菌是引起泌尿生殖道感染的病原菌之一 ,其分离率以副流感嗜血杆菌为主 ,对其型别的检测有利于对菌株的流行病学调查 ;我国临床与实验室人员应加强对嗜血杆菌引起泌尿生殖道感染的重视。

Objective To investigate the distribution of H.Spp. in genitourinary infections.Methods A total of 5,022 genitourinary specimens from men and women were cultured and identified for H.spp. using a simple selective culture method (HAE) and API NH system.Results In the specimens, 198 strains (3.9%) were positive for H.Spp. , and H.parainfluenzae , H.influenzae , H.aphrophilus/paraphro. were 139 (70.2%), 54(27.3%) and 5(2.5%) strains respectively.The most frequent biotypes were H.parainluenzae biotype Ⅱ(29.3 % ), Ⅲ (24.8 % ) and H.influenzae biotype Ⅳ (19.3% ). The highest isolation rate of urethritis was 39.9% in men.Conclusions H.spp. is one of the pathogens to induce genitourinary infection. The isolation result of H.parainfluenzae is highest.Typing of H.spp. is useful for epidemiological investigation. It is necessary to pay more attention to culture of H.spp. in China.