互叶白千层水提物药理作用研究

Study on Pharmacological Action of Aqueous Extract from Melaleuca Ahemifolia

目的:观察互叶白千层水提物的药理作用。方法:采用巴豆油致小鼠耳廓肿胀法、微量2倍稀释法、小鼠热板法、小鼠被动皮肤过敏试验、豚鼠磷酸组胺致痒试验等试验方法观察互叶白千层水提物的药理作用,并对其进行急性毒性试验。结果:互叶白千层水提物能抑制巴豆油引起小鼠耳廓肿胀;提高小鼠热刺激疼痛的痛阈值;对金黄色葡萄球菌、表皮葡萄球菌、大肠埃希杆菌、白色念珠菌有较强抑菌作用;抑制小鼠被动皮肤通透性;增加豚鼠耐受磷酸组胺的致痒阈;互叶白千层水提物的最大给药量为225.62 g生药/kg。结论:互叶白千层水提物具有抗炎、镇痛、抗菌、抗过敏、止痒等药理作用,且毒性小。

Objective： To observe the pharmacological action of aqueous extract from Melaleuca Ahemifolia. Methods： The experiments concluded mice ear-swollen induced by croton oil, double broth dilution method, hot plate test, passive cutaneous anaphylaxis （PCA）in mouse, cavy itch test by histamine phosphate and acute toxicity test to observe the pharmacological action of aqueous extract from Melaleuca Ahemifolia. Results： Aqueous Extract from Melaleuca Ahemifolia can inhibit the mice earswollen induced by croton oil, enhance the threshold of pain in mice due to hot stimulation, provide obvious bacteriostatic action on staphylococcus aureus, staphylococcus epidermidis, bacillus coli and albicans Saccharomyces, reduce the PCA and improve cavy itch-threshold by histamine phosphate. The acute toxicity test showed that maximum tolerance of water extract of melaleuca ahernifolia was 225.62 g crude drug/kg. Conclusion： Aqueous Extract from Melaleuca Ahemifolia can provide obvious anti-inflammatory, analgesic, antimicrobial, anti-allergic and antipruritic effects, with low toxicity.