槲皮素对人肝癌SMMC-7721细胞增殖和凋亡及其凋亡通路的影响

Study on Influence of Quercetin on Proliferation,Apoptosis and Apoptotic Pathway of Human Hepatoma SMMC-7721 Cells

目的:研究槲皮素体外对人肝癌细胞凋亡及其凋亡通路的影响。方法:体外培养人肝癌SMMC-7721细胞,采用MTT法检测细胞增殖;采用流式细胞术检测凋亡率和细胞周期;应用ELISA法检测细胞上清液中Caspase-3、Caspase-9及Survivin的含量。结果:槲皮素(10-320μmol.L-1)体外可明显抑制SMMC-7721细胞的增殖,并具有浓度和时间依耐性;在40-160μmol.L-1浓度下,槲皮素可诱导SMMC-7721细胞凋亡;将细胞阻滞在G2-M期;使Caspase-3、Caspase-9蛋白表达量显著增加,而Survivin蛋白表达显著减少。结论:槲皮素抑制人肝癌SMMC-7721细胞的作用机制与诱导细胞凋亡和将细胞周期阻滞在G2-M期有关,其凋亡通路涉及Caspase途径。

Objective： To study the influence on apoptosis and apoptosis pathway in human hepatoma SMMC-7721 cells by quercetin. Methods： SMMC-7721 cells were cultured in vitro, and MTF was performed to observe the proliferation. Flow cytometry was utilized for measure the apoptosis and cell cycle. The expressions of Caspase-3, Caspase-9 and Survivin in cultured supernatant were detected by ELISA assay. Results： Quercetin could inhibit the proliferation of SMMC-7721 cells in vitro at l0 to 320 μmol·L-1, which was concentration- and time-dependent. At a dosage between 40 and 160μmol·L-1 in cultured ceils, the apoptosis rates was elevated, and cell cycle was blocked in G2-M phase. Quercetin was able to increase the expressions of Caspase-3 and Caspase-9 proteins but decrease the expression of Survivin protein. Conclusion： Quereetin can inhibit the proliferation of SMMC-7721 cells via the apoptosis induction and cell cycle inhibition, moreover, Caspase pathway was involved in the apoptosis pathway.