摘要
目的 检测肾综合征出血热病毒 (HFRSV)在革螨、恙螨体内生存空间与时间。方法 采集自然界鼠窝革螨饲养繁殖的子代革螨和鼠体恙螨幼虫、小黑板采集的游离恙螨幼虫及饲养孵化的若虫 ,进行石腊包埋切片 ,接种Vero E6细胞培养 ,应用IFAT检测分别观察HFRSV在螨体内分布和测定不同时期HFRSV的滴度。结果 发现HFRSV多分布于螨体的腹部组织即消化器官 ,前体部分较少 ,个别螨的前足和中足亦可检测到 ;经TCID50 /ml滴度测定以 10 0天为最高 (10 5～ 6)。结论 表明HFRSV在螨体内存在时间较长 ,并可经期传递 ;
S:Aim\ In order to study the distribution and life span of hemorrhagic fever with renal syndrome virus (HFRSV) in Gasmid mite and Bhigger mite, Methods\ Paraffin sections of larva from mice, free larva, reared larva,adult and inoculated Vero-E\-6 culture were examined by IFAT. Results\ The results showed that HFRSV were dispersed on belly, and only a few on front. HFRSV were found also in front leg and separated from larva and adult during different time. The titer of TCID50/ml was the highest(10 5-6 )after inoculated 100 days. Conclusion\ It demonstrates that HFRSV can stay in mites for a long time and be transmitted from stage to stage .
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第5期5-7,共3页
Chinese Journal of Zoonoses
基金
国家自然科学基金资助项目!(N:39970653)
