高迁移率族蛋白HMGA1在肝细胞癌及胆管细胞癌中的表达差异及临床意义

Different expression of HMGA1 in hepatocarcinoma and intrahepatic cholangiocarcinoma and its significance

目的探讨高迁移率族蛋白A1(HMGA1)在肝细胞癌(HCC)、肝内胆管细胞癌(IHC)中的表达差异和临床意义。方法采用免疫组化SABC法对60例HCC、42例胆管细胞癌及10例正常肝脏组织标本中HMGA1的表达情况进行检测,并分析其与临床病理特点之间的关系。结果 HMG组A1在10例正常肝脏组织中无一例表达,在HCC组中表达率为3.3%(2/60),在IHC组中表达率为66.7%(28/42)。IHC组与HCC组中HMGA1表达差异有统计学意义(P=0.000);IHC组HMGA1与p53的表达呈正相关(r=0.673,P<0.05);1年内死亡患者的HMGA1表达水平(80.0%)较1年内存活患者的(33.3%)高,差异有统计学意义(P<0.05)。结论 HMGA1可作为鉴别肝细胞癌与肝内胆管细胞癌的一个潜在指标。HMGA1在肝内胆管细胞癌的生成中可能发挥了重要作用,HMGA1在肝内胆管细胞癌的高表达提示预后不良。

Objective：To investigate the expression of HMGA1 in hepatocellular carcinoma （HCC） and intrahepatic cholangiocarcinoma（IHC） and its significance. Methods：Immunohistochemistry （SABC method） was applied to detect HMGA1 expression in 60 HCC, 42 IHC, and 10 normal hepatic tissues. The relationship between HMGA1 expresssion and clinicpathological features of HCC and IHC was determined. Results：No significant nuclear immunoreactivity was found in normal hepatic tissues, while 3.3% （2/60） of HCC, 66.7% （28/42） of IHC showed positive immunoreactivity, and there was significant difference between HCC and IHC（P = 0.000）. The expression level of HMGA1 was strongly correlated with the expression level of p53 （r = 0.673, P 〈 0.05）. The expression of HMGA1 in patients who died in one year （80.0%） was higher than that of patients lived（33.3%）, and the difference was significant（P 〈 0.05）. Conclusions：HMGA1 protein level may serve as a potential diagnostic marker that differentiate HCC from IHC. HMGA1 may play an important role in the development of IHC. The high expression level of HMGA1 in IHC is strongly correlated with bad prognosis.