趋化因子CXCL5在肝癌中的表达及其临床意义

Expression of CXCL5 in primary hepatocellular carcinoma and its significance

目的探讨趋化因子配体5(CXCL5)在原发性肝细胞肝癌(HCC)中的表达,分析其在肝癌发生、发展、侵袭及转移中的作用。方法收集218例HCC组织标本及其临床病理资料,采用免疫组化(IHC)Elvision检测肝癌组织、癌旁组织、周围正常组织中CXCL5的表达,染色后阅片、评分、对比其在各组之间的表达差异;采用相关分析、非参数检验、Log-Rank、Cox回归分析和Logistic回归分析进行统计学分析,P<0.05认为有统计学意义。结果 IHC结果显示CXCL5在癌组织中的阳性率高于癌旁组织(P<0.01)和周围正常组织(P<0.01);癌组织CXCL5中高表达与门静脉癌栓形成、BCLC分期、TNM分期、肝癌并静脉侵犯正相关,与病理分级负相关;CXCL5是肝癌患者的独立预后因素之一(HR1.734;P=0.001)。结论 CXCL5在HCC中表达增高,与肝癌的病理分级、恶性程度、门静脉癌栓、临床分期呈正相关,其高表达是HCC的独立预后危险因子之一。

Objective：To explore the expression of CXCL5 in resectable human hepatocellular carcioma（HCC） and evaluate the role of CXCL5 in the pathogenesis, proliferation, invasion and metastasis of HCC. Methods：Collect the tissue specimen of HCC patients after resection and their clinicopathological data. The expression of CXCL5 in tumor, adjacent-tumor tissues and corresponding normal liver tissue were detected by immunohistochemistry（IHC） Elvision method. Results：The IHC detection of CXCL5 indicates that the rate of positive of staining of CXCL5 was significantly higher than that in adjacent-tumor tissue （P 〈 0.01） and corresponding normal tissue （P 〈 0.01）. The expression of CXCL5 was positive correlated significantly with portal vein tumor invasion, BCLC staging and TNM staging, and negative correlation with pathological grades. Cox analysis identified the high expression of CXCL5 as one of the independent risk factors predictive of poor prognosis （HR 1.734, P = 0.001）. Conclusions：The expression and concentration of CXCL5 were higher in HCC. They associated with pathological grade, portal vein tumor thrombosis, progression of tumor and clinical stage. High expression of CXCL5 is one of the independent risk factors predictive of poor prognosis.