发病罗非鱼苗沃氏葡萄球菌的分离与鉴定

Isolation and Identification of Staphylococci warneri from Diseased Tilapia Fry

为检测发病罗非鱼苗中可能存在的潜在病原感染,保证投放鱼苗的健康安全,对海口市发病罗非鱼苗进行了菌种分离。结果发现,在发病鱼苗中存在葡萄球菌,经16S rRNA基因序列测定,显示该菌与沃氏葡萄球菌和巴斯德葡萄球菌高度相似,后经超氧(化)物歧化酶sodA基因PCR分析,确认为沃氏葡萄球菌。

Strains of Staphylococci,the largest Gram positive stains,were isolated by direct plating and Gram stain from the samples of livers and gallbladders from the diseased Tilapia fry collected in Haikou. Analysis of 16S rRNA gene sequence showed that these isolates were highly homologous with Staphylococcus pasteuri and Staphylococcus warneri,but further identified to be Staphylococcus warneri by PCR amplification of the sodA gene for superoxide dismutase.