与苦丁茶炭疽病抗性基因连锁的RAPD标记

RAPD Markers Linked to the Resistance Gene of Ilex kudingcha Against Colletotrichum illics-kudingchae

为了研发出与苦丁茶炭疽病抗性基因紧密连锁的RAPD-SCAR特异标记,本研究中首先利用92条RAPD随机引物对苦丁茶冬青中已知对炭疽病高抗或高感的不同种质材料进行RAPD分析,并从中寻找到4个与苦丁茶炭疽病抗性基因紧密连锁的RAPD特异标记特异性片段S69-300,S227-300,S227-2000和S247-400。后续的研究可对这些RAPD特异片段进行回收,扩增和测序,并将测序结果作为开发与苦丁茶炭疽病抗性基因紧密连锁的SCAR标记的基础。

In order to develop specific SCAR markers closely linked to the gene resistant to Colletotrichum illics-kudingchae G. M. Liu et Z. Y. Zhang,germplasm materials of Ilex kudingcha with high resistance and high susceptibility to C. illics-kudingchae were collected for RAPD analysis with 92 random primers,from which a few specific RAPD markers closely linked to the resistance gene were amplified. These markers included the specific fragments： S69-300,S227-320,S227-2000 and S247-400. These specific fragments will be retrieved,amplified and sequenced. The specific RAPD fragment sequencing results can be used for development of SCAR markers linked to the resistance gene against C. illics-kudingchae.