影响木薯器官发生主要因素的优化

Optimization of Major Factors Influencing Cassava Organogenesis

为了给木薯主栽品种的转基因改良提供条件,对木薯4个主栽品种NZ188,SC8,C3及C4器官发生的影响因素(基因型,蔗糖,IBA,AgNO3)进行了优化研究。结果表明:在体细胞胚的发生、器官发生等能力上,不同基因型之间存在明显差异,NZ188,SC8和C3在黑暗条件下侧芽膨大,初级体细胞胚的诱导率可达80%以上,而C4品种则在光照培养下效果较好;在进行体细胞胚诱导和成熟子叶胚的诱导时,培养基中蔗糖质量浓度为25～30g·L-1时效果最好;不定芽诱导培养基中添加0.3mg·L-1的IBA及4mg·L-1的AgNO3时,不定芽的诱导率及芽再生数目明显提高。

Factors （ genotypes,sucrose,IBA and AgNO3 ） affecting organogensis of 4 cassava cultivars （ NZ188, SC8,C3 and C4） were analyzed and optimized for genetic improvement. The results showed significant differences in somatic embryogenesis and organogenesis among the genotypes tested. Axillary buds of the cultivars NZ188,SC8 and C3 swelled in the dark condition and their primary somatic embryos had an inducing frequency of more than 80% ,while C4 grew better in the light condition. Somatic embryogenesis and mature somatic embryos were induced best on the medium containing 25—30 g·L-1 sucrose. The inducing frequency of adventitious shoots and the shoot regeneration rate were improved significantly on the shoot organogenesis medium at the presence of 4mg·L-1 AgNO3 and 0.3 mg·L-1 IBA.