光裸方格星虫人工繁殖及苗种培育技术

Artificial Propagation and Seed Breeding Technology of Sipunculus nudus

对光裸方格星虫(Sipunculus nudus)亲虫培育习性进行观察、人工催产及育苗研究。结果表明,光裸方格星虫在自然海区分布的雌雄比例约为1∶1.1;其亲虫在中细沙质(粒径0.1～2.0mm)底质环境下培育的成活率最高;采用阴干刺激3h+流水刺激+精液诱导相结合的诱导方法进行催产,催产效果最佳;胚胎发育在26.5～31.5℃的范围时,随着温度的升高,受精卵孵化出初期海球幼虫所需的时间逐渐变短。人工育苗试验共获得光裸方格星虫苗15.2万条,平均体长2.8cm。

Observation on the maternal culture,artificial spawning and breeding of marine worm Sipunculus nudus were conducted. The marine worm was distributed in the natural sea waters at the ratio of the male to female of about 1∶1.1,and the survival rate of the maternal marine worms was the highest when cultured in fine sand （ 0.1-2.0 mm） . Maternal marine worms spawned best when forced by a combined spawning method （ drying in the air for 3 hours ＋ running water stimulating ＋ semen inducing） as compared with other different artificial spawning methods. When the temperature for embryonic development fell in the range of 26. 5 ℃ to 31. 5 ℃ , the initial time for larvae to hatch from the fertilized eggs was reduced as the temperature increased. Our artificial breeding tests had produced a total of 152 000 marine worms with an average body size of 2. 8 cm long.