培哚普利对慢性心功能不全大鼠心肌肌浆网Ca^(2+)泵功能的影响

Effect of peridopril on myocardial sarcoplasmic reticulum Ca^(2+)pump in rats with chronic cardiac insufficiency

目的 探讨血管紧张素转换酶抑制剂 (ACEI)干预慢性心功能不全对心肌肌浆网 (SR)Ca2 + 泵活性和Ca2 + 摄取功能的影响及意义。方法 80只大鼠分为心功能不全组 (F组 ) ,培哚普利组 (P组 ) ,对照组 (C组 )。通过结扎大鼠左冠脉建立慢性心功能不全模型 ,以培哚普利进行干预 ,对照观察血流动力学、左室心肌SRCa2 + 泵活性、SRCa2 + 摄取Vmax、Kd 值和最大摄取量。结果 F组左室舒张末压 (LVEDP)显著升高 (P <0 .0 1) ,+dp dtmax、-dp dtmax显著降低 (P <0 .0 1) ;P组LVEDP显著低于F组 (P <0 .0 1) ,+dp dtmax、-dp dtmax显著高于F组 (P <0 .0 1)。F组心肌SRCa2 + 泵活性、Ca2 + 最大摄取量和Vmax显著低于C组 (P <0 .0 1) ,P组显著高于F组 (P <0 .0 1) ,3组Kd 值差异不显著 (P >0 .0 5 )。心肌SRCa2 + 泵活性与Vmax、+dp dtmax、-dp dtmax显著正相关 (r=0 .742 9,0 .5 161,0 .6172 ,P <0 .0 1)。结论 培哚普利长期干预慢性心功能不全 ,能够增加心肌SRCa2 + 泵活性 ,改善Ca2 + 摄取功能 ,可能与其改善心肌舒缩功能及心肌保护作用有关。

Objective To study the effects and significance of angiotensin converting enzyme inhibitor (ACEI) on Ca 2+ pump activity and Ca 2+ uptake function in myocardial sarcoplasmic reticulum (SR) in the prevention and treatment of chronic cardiac insufficiency. Methods The models of chronic cardiac insufficiency were established with left coronary ligation in 60 rats and then they were divided into group F(Cardiac insufficiency) and group P(Peridopril treated). Other 20 rats were employed as control group. Hemodynamic parameters, SR Ca 2+ pump activity of left ventricle, Vmax and maximum amount of Ca 2+ uptake by SR, and Kd of Ca 2+ uptake were determined. Results Left ventricular end diastolic pressure (LVEDP) was increased significantly in group F than in control (P<0.01) but +dp/dt max and -dp/dt max was decreased obviously (P<0.01). LVEDP was markedly lower in group P than in group F (P<0.01) but +dp/dt max and -dp/dt max was remarkably higher (P<0.01). SR Ca 2+ pump activity, Vmax and maximum amount of Ca 2+ uptake were highest in group P, higher in group F and lowest in control group (P<0.01). But no significant difference was found in K d of the 3 groups. Myocardial SR Ca 2+ pump activity was positively correlated with +dp/dt max and -dp/dt max significantly (r=0.7429, 0.5161 ,0.6172, P<0.01). Conclusion Peridopril can improve the Ca 2+ pump activity and Ca 2+ uptake function of myocardial SR in the prevention and treatment of chronic cardiac insufficiency, which might due to its enhancement to myocardial function and protection.