HCV感染外周血单个核细胞及其对T淋巴细胞亚群的影响

Infection of peripheral blood mononuclear cells by hepatitis C virus and the influence on T lymphocyte subset

目的 :研究丙型肝炎病毒 (HCV)感染外周血单个核细胞 (PBMC)的情况及其对T淋巴细胞亚群的影响。方法 :运用非同位素原位杂交 (NISH)法和链酶亲和素 生物素 (SABC)法分别检测 2 0例慢性丙型肝炎患者PBMC中的HCV RNA和非结构 (Nonstructural,NS)蛋白NS5抗原 ,同时用SABC法检测其T淋巴细胞亚群。结果 :8例 (4 0 0 % )患者的PBMC中HCV RNA呈阳性 ,其中 6例 (30 0 % )患者的PBMC中NS5抗原同时呈阳性。HCV RNA和NS5抗原均呈散在颗粒状或弥漫分布 ,主要位于胞浆中 ,NS5抗原还出现于胞膜上。HCV RNA和NS5阳性细胞的比例均很低 ,分别为 0 1%～ 2 0 %和 0 1%～ 3 0 %。HCV RNA阳性组和阴性组的CD4+T细胞的比例均低于正常对照组 ,CD8+T细胞比例高于正常组 ,CD4+/CD8+比值下降 ;而HCV RNA阳性组的CD4+T细胞比例则又低于阴性组 ,CD8+T细胞比例高于阴性组 ,CD4+/CD8+比值出现倒置 (P值均小于 0 0 1)。结论 :HCV可以感染慢性丙型肝炎患者的PBMC并在其中复制 ;HCV感染PBMC后引起CD4+T细胞比例减少 ,CD8+T细胞比例增加 ,导致CD4+/CD8+比值下降甚至倒置 。

Objective: To investigate peripheral blood mononuclear cells (PBMCs) infected by hepatitis C virus (HCV) and the influence on T lymphocyte subset Methods: Using non isotopic in situ hybridization (NISH) and streptadivin biotin enzyme complex assay (SABC) to detect HCV RNA, NS 5 antigen and T lymphocyte subset Results: 8(40 0%) out of 20 patients with hepatitis C virus were HCV RNA positive in PBMCs, and among them 6(30 0%) were NS 5 antigen positive simultaneously NS 5 antigen signals appeared diffusely within cytoplasm, or in cell membrane,while HCV RNA signals were mainly distributed in cytoplasm Both the proportion of NS 5 positive cell and HCV RNA positive cells were very low (≤3%).The proportion of positive cellsane, while HCV RNA signals are very low (≤3%) The proportion of CD4 + T cells was lower in the patients comparing with that in control group, the proportion of CD8 + T cells was higher, and the ratio of CD4 +/CD8 + were decreased remarkably (P<0 01) Moreover the proportion of CD4 + T cells was lower in HCV RNA positive group comparing with that in HCV RNA negative group, the proportion of CD8 + T cells was higher, and the ratio of CD4 +/CD8 + was reversed (P<0 01) Conclusion:HCV can infect PBMCs and replicate in patients with chronic hepatitis C;CD4 + T cells are readily damaged after PBMCs infected by HCV, which causes the decrease or reversal of the ratio of CD4 +/CD8 +, undermines the ability of the body clearing virus and results im chronicity of HCV infection