多肽疫苗CopI诱导的特异性免疫反应

Study on peptide vaccine Cop-I induces the specific immune response in MS patients

目的 :研究了经Cop I治疗后的多发性硬化 (MS)患者外周血淋巴细胞 (PBMC)对Cop I及碱性髓鞘蛋白 (MBP)免疫的反应性。方法 :用半有限稀释微量培养技术分析经Cop I治疗的MS患者的PBMC对Cop I和MBP诱导的特异性T细胞株的反应频率。用ELISA方法检测T细胞株分泌的细胞因子类型。用免疫荧光检测了特异性Cop I细胞株的表型。结果 :15位经Cop I治疗后的MS患者PBMC中的Cop I/MBP特异性T细胞克隆反应频率高达 84 0 % ,而在 9位非MS患者对照组中 ,其T细胞对Cop I/MBP反应的频率仅为 5 4%。用ELISA方法检测发现体外Cop I/MBP诱导的特异性T细胞株株分泌IL 5和IL 13 ,为Th2类型的细胞因子。所有的细胞株均表现为CD3阳性 ,大部分高表达CD8细胞并发现有CD3/CD5 6双阳性的NKT细胞。结论 :上述结果提示多肽疫苗Cop I能诱导MS患者特异性免疫反应 ,以诱导CD8阳性T细胞和NKT为特点 。

Objective:To study on the immune response of patients with MS received Cop I for one year.Methods:The frequency of Cop I/MBP reactive T cell line was detemined by split well microtitre method.The cytokine profile of these T cells was quantitatively analyzed by ELISA.The phenotype of these T cell lines was assessed by immunofluroscence technique.Results:In 15 MS patients and 9 other neurologic diseases(OND)patients who received the Cop I for one year,the mean frequency of Cop I/MBP specific T cell lines was 84 0% and 5.4% respectively.The cytokine profile of Cop I specific T cell lines demonstrated the Th2 cytokines like IL 5 and IL 13.Further,all of the MBP/Cop I specific T cell lines were CD3 positive Cop I,most of them expressed CD8 and some of them were CD3/CD56 double positive cells called NKT,which only kill the Glioma cells but leave the K562 alive.Conclusion:These results indicate that Cop I can induce the specific immune response involved in mainly by CD8 and NKT cells.Meanwhile Cop I can upregulate Th2 cell in the T cell immune repertorie in the Cop I treating MS patients.